A metastatic model of human colon cancer constructed using cecal implantation of cancer tissue in nude mice

Toshiharu Furukawa, Tetsuro Kubota, Masahiko Watanabe, Tsong Hong Kuo, Hideki Nishibori, Suguru Kase, Yoshiro Saikawa, Hirokazu Tanino, Tatsuo Teramoto, Kyuya Ishibiki, Masaki Kitajima

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

COL-2-JCK, a human colon cancer xenograft line able to be transplanted into nude mice, was implanted in the subserosal layer of the cecum, either as cancer tissue or as a single cell suspension. When cancer tissue was used for the cecal implantation, 100% extensive local tumor growth and a high incidence of metastases to the regional lymph nodes, peritoneum, liver, and lung was observed. In contrast, when the cell suspension of this line was injected into the cecal wall, no metastases were observed, with significantly reduced local tumor growth. The use of cancer tissue maintaining the original cancer tissue structure is therefore considered imperative for allowing full expression of the biological characteristics of cancer cells. This nude mouse model using the cecal implantation of cancer tissue should thus prompt further study on the biology of human colon cancer.

Original languageEnglish
Pages (from-to)420-423
Number of pages4
JournalSurgery Today
Volume23
Issue number5
DOIs
Publication statusPublished - 1993 May

Fingerprint

Cecal Neoplasms
Nude Mice
Colonic Neoplasms
Neoplasms
Suspensions
Neoplasm Metastasis
Cecum
Peritoneum
Growth
Heterografts
Lymph Nodes
Cell Line
Lung

Keywords

  • human colon cancer
  • metastases
  • nude mouse

ASJC Scopus subject areas

  • Surgery

Cite this

A metastatic model of human colon cancer constructed using cecal implantation of cancer tissue in nude mice. / Furukawa, Toshiharu; Kubota, Tetsuro; Watanabe, Masahiko; Kuo, Tsong Hong; Nishibori, Hideki; Kase, Suguru; Saikawa, Yoshiro; Tanino, Hirokazu; Teramoto, Tatsuo; Ishibiki, Kyuya; Kitajima, Masaki.

In: Surgery Today, Vol. 23, No. 5, 05.1993, p. 420-423.

Research output: Contribution to journalArticle

Furukawa, T, Kubota, T, Watanabe, M, Kuo, TH, Nishibori, H, Kase, S, Saikawa, Y, Tanino, H, Teramoto, T, Ishibiki, K & Kitajima, M 1993, 'A metastatic model of human colon cancer constructed using cecal implantation of cancer tissue in nude mice', Surgery Today, vol. 23, no. 5, pp. 420-423. https://doi.org/10.1007/BF00309500
Furukawa, Toshiharu ; Kubota, Tetsuro ; Watanabe, Masahiko ; Kuo, Tsong Hong ; Nishibori, Hideki ; Kase, Suguru ; Saikawa, Yoshiro ; Tanino, Hirokazu ; Teramoto, Tatsuo ; Ishibiki, Kyuya ; Kitajima, Masaki. / A metastatic model of human colon cancer constructed using cecal implantation of cancer tissue in nude mice. In: Surgery Today. 1993 ; Vol. 23, No. 5. pp. 420-423.
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AU - Kase, Suguru

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