A method for determination of aldosterone in adrenal tributary venous serum by derivatization using Girard P reagent isotopologues followed by LC/ESI-MS/MS

Tatsuya Higashi, Miho Akaishi, Mai Yokota, Tatsunori Suzuki, Shoujiro Ogawa, Yuki Sugiura, Tetsuo Nishikawa, Koshiro Nishimoto, Makoto Suematsu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The quantification of aldosterone (ALD) in adrenal tributary venous blood serum/plasma combined with the super-selective adrenal venous sampling (ssAVS) technique is recognized as a definitive procedure for differentiation of the forms of primary aldosteronism (PA), identification of the affected segment(s) and operating decision-making. In this study, an enhanced throughput and sensitive method was developed and validated for the quantification of ALD in ssAVS serum samples by liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) combined with derivatization using the Girard P reagent (GP) isotopologues (2H0- and 2H5-GP). The right and left adrenal serum samples were separately pretreated and derivatized with either isotopologue. The two samples were then combined and injected together for LC/ESI-MS/MS analysis. Based on the mass differences between the isotopologues, the derivatized ALD in the two samples were quantified within a single run. This method enabled the reproducible (intra- and inter-assay relative standard deviations, 6.6% or lower) and accurate (98.2–107.0%) quantification of the serum ALD using a 25-μL sample, and the lower limit of quantification was 1.0 ng/mL. The developed method was used for the analysis of 11 pairs of ssAVS serum samples (total of 22 samples) of patients with ALD-producing adenoma and proven to have a satisfactory applicability; this method enabled the identification of the affected adrenal and the determination of the laterality of PA, and reduced the analysis time to about 2/3 compared to the previous method for 22 samples.

Original languageEnglish
Pages (from-to)106-113
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1092
DOIs
Publication statusPublished - 2018 Aug 15

Fingerprint

Aldosterone
Serum
Sampling
Hyperaldosteronism
Assays
Electrospray ionization
Liquid chromatography
Electrospray Ionization Mass Spectrometry
Mass spectrometry
Tandem Mass Spectrometry
Blood
Liquid Chromatography
Adenoma
Decision making
Throughput
Girard's reagent T
Decision Making
Plasmas

Keywords

  • Aldosterone
  • Analysis throughput
  • Isotopologue
  • LC/ESI-MS/MS
  • Super-selective adrenal venous sampling

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

A method for determination of aldosterone in adrenal tributary venous serum by derivatization using Girard P reagent isotopologues followed by LC/ESI-MS/MS. / Higashi, Tatsuya; Akaishi, Miho; Yokota, Mai; Suzuki, Tatsunori; Ogawa, Shoujiro; Sugiura, Yuki; Nishikawa, Tetsuo; Nishimoto, Koshiro; Suematsu, Makoto.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 1092, 15.08.2018, p. 106-113.

Research output: Contribution to journalArticle

Higashi, Tatsuya ; Akaishi, Miho ; Yokota, Mai ; Suzuki, Tatsunori ; Ogawa, Shoujiro ; Sugiura, Yuki ; Nishikawa, Tetsuo ; Nishimoto, Koshiro ; Suematsu, Makoto. / A method for determination of aldosterone in adrenal tributary venous serum by derivatization using Girard P reagent isotopologues followed by LC/ESI-MS/MS. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2018 ; Vol. 1092. pp. 106-113.
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AU - Yokota, Mai

AU - Suzuki, Tatsunori

AU - Ogawa, Shoujiro

AU - Sugiura, Yuki

AU - Nishikawa, Tetsuo

AU - Nishimoto, Koshiro

AU - Suematsu, Makoto

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