A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed

Junya Kobayashi, Hisashi Shidara, Yuma Morisawa, Maki Kawakami, Yuta Tanahashi, Kohji Hotta, Kotaro Oka

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Specific neuron ablation with laser microbeam has been used in behavioral analysis of Caenorhabditis elegans. However, this method is hard to acquire many ablated worms, and is unable to compare behavioral changes just before and after ablation. Here, we developed an ablation method by using genetically encoded photosensitizer protein, KillerRed, which produces reactive oxygen species by green light irradiation. Ablation of AWA sensory neurons abolished the chemotaxis to AWA specific sensitive attractant, diacetyl, and no functional effect on the other sensory neuron, AWC, which senses benzaldehyde. This ablation method can be useful for analyzing neural in situ.

Original languageEnglish
Pages (from-to)261-264
Number of pages4
JournalNeuroscience Letters
Volume548
DOIs
Publication statusPublished - 2013 Aug 26

Keywords

  • C. elegans
  • Chemosensation
  • KillerRed
  • Neural dynamics
  • Photo-inactivation
  • Reactive oxygen species

ASJC Scopus subject areas

  • Neuroscience(all)

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