TY - JOUR
T1 - A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed
AU - Kobayashi, Junya
AU - Shidara, Hisashi
AU - Morisawa, Yuma
AU - Kawakami, Maki
AU - Tanahashi, Yuta
AU - Hotta, Kohji
AU - Oka, Kotaro
N1 - Funding Information:
We thank Prof. Y. Kohara of National Institute of Genetics for providing plasmid odr-10p::GFP. This work is supported by Grants-in-Aid for Scientific Research ( 22650062 , 24650159 and S0801008 ).
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/8/26
Y1 - 2013/8/26
N2 - Specific neuron ablation with laser microbeam has been used in behavioral analysis of Caenorhabditis elegans. However, this method is hard to acquire many ablated worms, and is unable to compare behavioral changes just before and after ablation. Here, we developed an ablation method by using genetically encoded photosensitizer protein, KillerRed, which produces reactive oxygen species by green light irradiation. Ablation of AWA sensory neurons abolished the chemotaxis to AWA specific sensitive attractant, diacetyl, and no functional effect on the other sensory neuron, AWC, which senses benzaldehyde. This ablation method can be useful for analyzing neural in situ.
AB - Specific neuron ablation with laser microbeam has been used in behavioral analysis of Caenorhabditis elegans. However, this method is hard to acquire many ablated worms, and is unable to compare behavioral changes just before and after ablation. Here, we developed an ablation method by using genetically encoded photosensitizer protein, KillerRed, which produces reactive oxygen species by green light irradiation. Ablation of AWA sensory neurons abolished the chemotaxis to AWA specific sensitive attractant, diacetyl, and no functional effect on the other sensory neuron, AWC, which senses benzaldehyde. This ablation method can be useful for analyzing neural in situ.
KW - C. elegans
KW - Chemosensation
KW - KillerRed
KW - Neural dynamics
KW - Photo-inactivation
KW - Reactive oxygen species
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U2 - 10.1016/j.neulet.2013.05.053
DO - 10.1016/j.neulet.2013.05.053
M3 - Article
C2 - 23748043
AN - SCOPUS:84880135868
SN - 0304-3940
VL - 548
SP - 261
EP - 264
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -