A method for the rapid discovery of naturally occurring products by proteins immobilized on magnetic beads and reverse affinity chromatography

Midori A. Arai, Eiji Kobatake, Takashi Koyano, Thaworn Kowithayakorn, Shigeaki Kato, Masami Ishibashi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A highly efficient screening method for naturally occurring products that bind to a specific target protein was demonstrated by using hVDR magnetic beads. The native ligand 1α, 25(OH)2 VD3 (1) was selectively bound by hVDR magnetic beads when present in a mixture of natural compounds. Furthermore, this method was shown to be applicable to the identification of natural products that interact with a specific protein immobilized on the beads from an extract of a natural resource. Two new natural compounds were isolated by this method. This approach will be helpful for the discovery of novel, naturally occurring products that bind to specific target proteins. This method has the further advantages that it can identify the HPLC peak corresponding to the target compound for isolation, as well as provide important UV, CD, or MS profile information.

Original languageEnglish
Pages (from-to)1802-1808
Number of pages7
JournalChemistry - An Asian Journal
Volume4
Issue number12
DOIs
Publication statusPublished - 2009 Dec 1
Externally publishedYes

Keywords

  • Drug discovery
  • Liquid chromatography
  • Magnetic beads
  • Natural products
  • Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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