A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents a microfluidic three-dimensional (3D) platform to investigate behaviors of glioma-initiating cells (GIC) cocultured with human umbilical vein endothelial cells (HUVEC). In this platform, these cells were cultured in microenvironments similar to in vivo. Especially, our experiments have been performed with GIC established by gene transfection [1], which is different from conventional tumor cell lines. Our results demonstrate that biochemical factors secreted by HUVEC attracts glioma invasion.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1075-1077
Number of pages3
Volume2
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Endothelial cells
Microfluidics
Assays
Cells
Tumors
Genes
Experiments

Keywords

  • Glioma-initiating cells
  • Invasion
  • Microfluidic device

ASJC Scopus subject areas

  • Bioengineering

Cite this

Fujioka, S., Sampetrean, O., Saya, H., & Sudo, R. (2013). A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 2, pp. 1075-1077). Chemical and Biological Microsystems Society.

A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. / Fujioka, Shingo; Sampetrean, Oltea; Saya, Hideyuki; Sudo, Ryo.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. p. 1075-1077.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fujioka, S, Sampetrean, O, Saya, H & Sudo, R 2013, A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 2, Chemical and Biological Microsystems Society, pp. 1075-1077, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.
Fujioka S, Sampetrean O, Saya H, Sudo R. A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2. Chemical and Biological Microsystems Society. 2013. p. 1075-1077
Fujioka, Shingo ; Sampetrean, Oltea ; Saya, Hideyuki ; Sudo, Ryo. / A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. pp. 1075-1077
@inproceedings{cfe095982b4447cd8e7953193ab97c38,
title = "A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture",
abstract = "This paper presents a microfluidic three-dimensional (3D) platform to investigate behaviors of glioma-initiating cells (GIC) cocultured with human umbilical vein endothelial cells (HUVEC). In this platform, these cells were cultured in microenvironments similar to in vivo. Especially, our experiments have been performed with GIC established by gene transfection [1], which is different from conventional tumor cell lines. Our results demonstrate that biochemical factors secreted by HUVEC attracts glioma invasion.",
keywords = "Glioma-initiating cells, Invasion, Microfluidic device",
author = "Shingo Fujioka and Oltea Sampetrean and Hideyuki Saya and Ryo Sudo",
year = "2013",
language = "English",
isbn = "9781632666246",
volume = "2",
pages = "1075--1077",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture

AU - Fujioka, Shingo

AU - Sampetrean, Oltea

AU - Saya, Hideyuki

AU - Sudo, Ryo

PY - 2013

Y1 - 2013

N2 - This paper presents a microfluidic three-dimensional (3D) platform to investigate behaviors of glioma-initiating cells (GIC) cocultured with human umbilical vein endothelial cells (HUVEC). In this platform, these cells were cultured in microenvironments similar to in vivo. Especially, our experiments have been performed with GIC established by gene transfection [1], which is different from conventional tumor cell lines. Our results demonstrate that biochemical factors secreted by HUVEC attracts glioma invasion.

AB - This paper presents a microfluidic three-dimensional (3D) platform to investigate behaviors of glioma-initiating cells (GIC) cocultured with human umbilical vein endothelial cells (HUVEC). In this platform, these cells were cultured in microenvironments similar to in vivo. Especially, our experiments have been performed with GIC established by gene transfection [1], which is different from conventional tumor cell lines. Our results demonstrate that biochemical factors secreted by HUVEC attracts glioma invasion.

KW - Glioma-initiating cells

KW - Invasion

KW - Microfluidic device

UR - http://www.scopus.com/inward/record.url?scp=84907379465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907379465&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84907379465

SN - 9781632666246

VL - 2

SP - 1075

EP - 1077

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

ER -