A microsensing system for the in vivo real-time detection of local drug kinetics

Genki Ogata, Yuya Ishii, Kai Asai, Yamato Sano, Fumiaki Nin, Takamasa Yoshida, Taiga Higuchi, Seishiro Sawamura, Takeru Ota, Karin Hori, Kazuya Maeda, Shizuo Komune, Katsumi Doi, Madoka Takai, Ian Findlay, Hiroyuki Kusuhara, Yasuaki Einaga, Hiroshi Hibino

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Real-time recording of the kinetics of systemically administered drugs in in vivo microenvironments may accelerate the development of effective medical therapies. However, conventional methods require considerable analyte quantities, have low sampling rates and do not address how drug kinetics correlate with target function over time. Here, we describe the development and application of a drug-sensing system consisting of a glass microelectrode and a microsensor composed of boron-doped diamond with a tip of around 40 μm in diameter. We show that, in the Guinea pig cochlea, the system can measure - simultaneously and in real time - changes in the concentration of bumetanide (a diuretic that is ototoxic but applicable to epilepsy treatment) and the endocochlear potential underlying hearing. In the rat brain, we tracked the kinetics of the drug and the local field potentials representing neuronal activity. We also show that the actions of the antiepileptic drug lamotrigine and the anticancer reagent doxorubicin can be monitored in vivo. Our microsensing system offers the potential to detect pharmacological and physiological responses that might otherwise remain undetected.

Original languageEnglish
Pages (from-to)654-666
Number of pages13
JournalNature Biomedical Engineering
Volume1
Issue number8
DOIs
Publication statusPublished - 2017 Aug 1

Fingerprint

Pharmacokinetics
Kinetics
Pharmaceutical Preparations
Bumetanide
Microsensors
Diamond
Boron
Microelectrodes
Cochlea
Audition
Diuretics
Anticonvulsants
Doxorubicin
Hearing
Glass
Rats
Epilepsy
Diamonds
Brain
Guinea Pigs

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Computer Science Applications

Cite this

Ogata, G., Ishii, Y., Asai, K., Sano, Y., Nin, F., Yoshida, T., ... Hibino, H. (2017). A microsensing system for the in vivo real-time detection of local drug kinetics. Nature Biomedical Engineering, 1(8), 654-666. https://doi.org/10.1038/s41551-017-0118-5

A microsensing system for the in vivo real-time detection of local drug kinetics. / Ogata, Genki; Ishii, Yuya; Asai, Kai; Sano, Yamato; Nin, Fumiaki; Yoshida, Takamasa; Higuchi, Taiga; Sawamura, Seishiro; Ota, Takeru; Hori, Karin; Maeda, Kazuya; Komune, Shizuo; Doi, Katsumi; Takai, Madoka; Findlay, Ian; Kusuhara, Hiroyuki; Einaga, Yasuaki; Hibino, Hiroshi.

In: Nature Biomedical Engineering, Vol. 1, No. 8, 01.08.2017, p. 654-666.

Research output: Contribution to journalArticle

Ogata, G, Ishii, Y, Asai, K, Sano, Y, Nin, F, Yoshida, T, Higuchi, T, Sawamura, S, Ota, T, Hori, K, Maeda, K, Komune, S, Doi, K, Takai, M, Findlay, I, Kusuhara, H, Einaga, Y & Hibino, H 2017, 'A microsensing system for the in vivo real-time detection of local drug kinetics', Nature Biomedical Engineering, vol. 1, no. 8, pp. 654-666. https://doi.org/10.1038/s41551-017-0118-5
Ogata, Genki ; Ishii, Yuya ; Asai, Kai ; Sano, Yamato ; Nin, Fumiaki ; Yoshida, Takamasa ; Higuchi, Taiga ; Sawamura, Seishiro ; Ota, Takeru ; Hori, Karin ; Maeda, Kazuya ; Komune, Shizuo ; Doi, Katsumi ; Takai, Madoka ; Findlay, Ian ; Kusuhara, Hiroyuki ; Einaga, Yasuaki ; Hibino, Hiroshi. / A microsensing system for the in vivo real-time detection of local drug kinetics. In: Nature Biomedical Engineering. 2017 ; Vol. 1, No. 8. pp. 654-666.
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