A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Tanya Barrett, Tao Xie, Yulan Piao, Ora Dillon-Carter, George J. Kargul, Meng K. Lim, Francis J. Chrest, Robert Wersto, Daniel L. Rowley, Magdalena Juhaszova, Li Zhou, Marquis P. Vawter, Kevin G. Becker, Christopher Cheadle, William H. Wood, Una D. McCann, William J. Freed, Minoru Ko, George A. Ricaurte, David M. Donovan

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

Original languageEnglish
Pages (from-to)822-833
Number of pages12
JournalNeurobiology of Disease
Volume8
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Methamphetamine
Dopaminergic Neurons
Oligonucleotide Array Sequence Analysis
Gene Library
Transcriptome
Gene Expression
Tyrosine 3-Monooxygenase
Electron Transport Complex IV
Substantia Nigra
Transgenic Mice
Neurotransmitter Agents
Molecular Biology
Up-Regulation
Complementary DNA
Clone Cells
Peptides
Brain
Population

Keywords

  • cDNA library
  • cDNA microarray
  • Development
  • Dopamine
  • Gene expression profile
  • Methamphetamine

ASJC Scopus subject areas

  • Neurology

Cite this

Barrett, T., Xie, T., Piao, Y., Dillon-Carter, O., Kargul, G. J., Lim, M. K., ... Donovan, D. M. (2001). A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity. Neurobiology of Disease, 8(5), 822-833. https://doi.org/10.1006/nbdi.2001.0423

A murine dopamine neuron-specific cDNA library and microarray : Increased COX1 expression during methamphetamine neurotoxicity. / Barrett, Tanya; Xie, Tao; Piao, Yulan; Dillon-Carter, Ora; Kargul, George J.; Lim, Meng K.; Chrest, Francis J.; Wersto, Robert; Rowley, Daniel L.; Juhaszova, Magdalena; Zhou, Li; Vawter, Marquis P.; Becker, Kevin G.; Cheadle, Christopher; Wood, William H.; McCann, Una D.; Freed, William J.; Ko, Minoru; Ricaurte, George A.; Donovan, David M.

In: Neurobiology of Disease, Vol. 8, No. 5, 2001, p. 822-833.

Research output: Contribution to journalArticle

Barrett, T, Xie, T, Piao, Y, Dillon-Carter, O, Kargul, GJ, Lim, MK, Chrest, FJ, Wersto, R, Rowley, DL, Juhaszova, M, Zhou, L, Vawter, MP, Becker, KG, Cheadle, C, Wood, WH, McCann, UD, Freed, WJ, Ko, M, Ricaurte, GA & Donovan, DM 2001, 'A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity', Neurobiology of Disease, vol. 8, no. 5, pp. 822-833. https://doi.org/10.1006/nbdi.2001.0423
Barrett, Tanya ; Xie, Tao ; Piao, Yulan ; Dillon-Carter, Ora ; Kargul, George J. ; Lim, Meng K. ; Chrest, Francis J. ; Wersto, Robert ; Rowley, Daniel L. ; Juhaszova, Magdalena ; Zhou, Li ; Vawter, Marquis P. ; Becker, Kevin G. ; Cheadle, Christopher ; Wood, William H. ; McCann, Una D. ; Freed, William J. ; Ko, Minoru ; Ricaurte, George A. ; Donovan, David M. / A murine dopamine neuron-specific cDNA library and microarray : Increased COX1 expression during methamphetamine neurotoxicity. In: Neurobiology of Disease. 2001 ; Vol. 8, No. 5. pp. 822-833.
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