A nanocarbon film electrode as a platform for exploring DNA methylation

Dai Kato, Naoyuki Sekioka, Akio Ueda, Ryoji Kurita, Shigeru Hirono, Koji Suzuki, Osamu Niwa

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

We describe the quantitative nonlabel electrochemical detection of both cytosine (C) and methylcytosine (mC) in oligonucleotides using newly developed nanocarbon film electrodes. The film consists of nanocrystalline sp2 and sp3 mixed bonds formed by employing the electron cyclotron resonance (ECR) sputtering method. We successfully used this film to develop a simple electrochemical DNA methylation analysis technique based on the measurement of the differences between the oxidation currents of C and mC since our ECR nanocarbon film electrode can directly measure all DNA bases more quantitatively than conventional glassy carbon or boron-doped diamond electrodes. The excellent properties of ECR nanocarbon film electrodes result from the fact that they have a wide potential window while maintaining the high electrode activity needed to oxidize oligonucleotides electrochemically. Proof-of-concept experiments were performed with synthetic oligonucleotides including different numbers of C and mC. This film allowed us to perform both C- and mC-positive assays solely by using the electrochemical oxidation of oligonucleotides without bisulfite or labeling processes.

Original languageEnglish
Pages (from-to)3716-3717
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number12
DOIs
Publication statusPublished - 2008 Mar 26

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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  • Cite this

    Kato, D., Sekioka, N., Ueda, A., Kurita, R., Hirono, S., Suzuki, K., & Niwa, O. (2008). A nanocarbon film electrode as a platform for exploring DNA methylation. Journal of the American Chemical Society, 130(12), 3716-3717. https://doi.org/10.1021/ja710536p