A Neo-Esophagus Reconstructed by Cultured Human Esophageal Epithelial Cells, Smooth Muscle Cells, Fibroblasts, and Collagen

Koji Hayashi, Nobutoshi Ando, Soji Ozawa, Yuukou Kitagawa, Hirotaka Miki, Michio Sato, Masaki Kitajima

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The scientists involved in this study attempted to develop an artificial esophagus constructed of autologous cells grown by cell culture methods on an extracellular matrix. An artificial esophagus consisting of human esophageal epithelial cells, dermal fibroblasts, and smooth muscle cells isolated from the aortic media, was attempted. The purpose of this study was to examine whether smooth muscle cells could be used in the transforming matrix. Human fibroblasts were embedded in Type I collagen superimposed on the collagen layer of smooth muscle cells. Next, human esophageal epithelial cells were cultured on the collagen layer of the fibroblasts. The resulting collagen sheets were cultured in vitro for 1 week, then transplanted on the latissimus dorsi muscles of athymic rats. The sheets were examined histologically at 1 and 2 weeks using hematoxylin eosin and immunologic stain methods (antiactin antibody). At the end of 2 weeks after transplantation, on microscopic observation of the collagen sheets, it appeared that the epithelial layer, the submucosal tissue layer, and the proper muscle layer had been reconstructed. Additionally, the authors successfully isolated smooth muscle cells from the media of the left gastric artery as a surgical specimen by explant cell culture. The ability to transform collagen sheets consisting of esophageal epithelial cells, fibroblasts, and smooth muscle cells from a surgical specimen into a luminal structure may enable clinical application of the artificial esophagus.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalASAIO Journal
Volume50
Issue number3
DOIs
Publication statusPublished - 2004 May

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Fibroblasts
Collagen
Esophagus
Smooth Muscle Myocytes
Muscle
Epithelial Cells
Cells
Cell Culture Techniques
Cell culture
Nude Rats
Muscles
Superficial Back Muscles
Hematoxylin
Eosine Yellowish-(YS)
Collagen Type I
Extracellular Matrix
Stomach
Coloring Agents
Arteries
Transplantation

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

A Neo-Esophagus Reconstructed by Cultured Human Esophageal Epithelial Cells, Smooth Muscle Cells, Fibroblasts, and Collagen. / Hayashi, Koji; Ando, Nobutoshi; Ozawa, Soji; Kitagawa, Yuukou; Miki, Hirotaka; Sato, Michio; Kitajima, Masaki.

In: ASAIO Journal, Vol. 50, No. 3, 05.2004, p. 261-266.

Research output: Contribution to journalArticle

Hayashi, Koji ; Ando, Nobutoshi ; Ozawa, Soji ; Kitagawa, Yuukou ; Miki, Hirotaka ; Sato, Michio ; Kitajima, Masaki. / A Neo-Esophagus Reconstructed by Cultured Human Esophageal Epithelial Cells, Smooth Muscle Cells, Fibroblasts, and Collagen. In: ASAIO Journal. 2004 ; Vol. 50, No. 3. pp. 261-266.
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