A neurospheroid cultured on the tip of a flexible microelectrode for cortical microstimulation

Keisuke Okita, Midori Kato-Negishi, Koji Sato, Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a flexible microelectrode combined with a neuronal spheroid (neurospheroid) cultured on the electrode tip. Prior works have found that neurons of a neurospheroid can extend their axons to form synaptic connections with neurons inside living neuronal tissues, that could enable such tissues to be stimulated via transplanted neurons (i.e., instead of through the insertion of invasive probes). Here we present a parylene-based flexible microelectrode that enables activation of neurospheroid cultured on the electrode. Experiments revealed that our probe successfully activated neurons within neurospheroids, resulting in neurite extension onto the electrode. These results mark an important step toward achieving minimally-invasive cortical microstimulation via neurospheroid-based probing systems.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1024-1026
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Microelectrodes
Neurons
Electrodes
Tissue
Chemical activation
Experiments

Keywords

  • Electrode
  • Microstimulation
  • Neural probe
  • Neurospheroid
  • Parylene

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Okita, K., Kato-Negishi, M., Sato, K., Onoe, H., & Takeuchi, S. (2012). A neurospheroid cultured on the tip of a flexible microelectrode for cortical microstimulation. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1024-1026). Chemical and Biological Microsystems Society.

A neurospheroid cultured on the tip of a flexible microelectrode for cortical microstimulation. / Okita, Keisuke; Kato-Negishi, Midori; Sato, Koji; Onoe, Hiroaki; Takeuchi, Shoji.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1024-1026.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Okita, K, Kato-Negishi, M, Sato, K, Onoe, H & Takeuchi, S 2012, A neurospheroid cultured on the tip of a flexible microelectrode for cortical microstimulation. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1024-1026, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.
Okita K, Kato-Negishi M, Sato K, Onoe H, Takeuchi S. A neurospheroid cultured on the tip of a flexible microelectrode for cortical microstimulation. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1024-1026
Okita, Keisuke ; Kato-Negishi, Midori ; Sato, Koji ; Onoe, Hiroaki ; Takeuchi, Shoji. / A neurospheroid cultured on the tip of a flexible microelectrode for cortical microstimulation. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1024-1026
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