A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing

Keita Nishiyama, Makoto Sugiyama, Hiroki Yamada, Kyoko Makino, Sayaka Ishihara, Takashi Takaki, Takao Mukai, Nobuhiko Okada

Research output: Contribution to journalArticle

Abstract

Several bacterial moonlighting proteins act as adhesion factors, which are important for bacterial colonization of the gastrointestinal (GI) tract. However, little is known about the adherence properties of moonlighting proteins in the GI tract. Here, we describe a new approach for visualizing the localization of moonlighting protein-coated fluorescent microbeads in the whole GI tract by using a tissue optical clearing method, using elongation factor Tu (EF-Tu) as an example. As a bacterial cell surface-localized protein mimic, recombinant EF-Tu from Lactobacillus reuteri was immobilized on microbeads. EF-Tu-coating promoted the interaction of the microbeads with a Caco-2 cell monolayer. Next, the microbeads were orally administered to mice. GI whole tissues were cleared in aqueous fructose solutions of increasing concentrations. At 1 h after administration, the microbeads were diffused from the stomach up to the cecum, and after 3 h, they were diffused throughout the intestinal tract. In the lower digestive tract, EF-Tu-beads were significantly more abundant than non-coated control beads, suggesting that EF-Tu plays an important role in the persistence of the microbeads in the GI tract. The new approach will help in evaluating how moonlighting proteins mediate bacterial colonization.

Original languageEnglish
Article number4731
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1
Externally publishedYes

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Bacterial Proteins
Peptide Elongation Factor Tu
Microspheres
Adhesives
Gastrointestinal Tract
Lactobacillus reuteri
Caco-2 Cells
Cecum
Fructose
Stomach
Membrane Proteins
Proteins

ASJC Scopus subject areas

  • General

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A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing. / Nishiyama, Keita; Sugiyama, Makoto; Yamada, Hiroki; Makino, Kyoko; Ishihara, Sayaka; Takaki, Takashi; Mukai, Takao; Okada, Nobuhiko.

In: Scientific reports, Vol. 9, No. 1, 4731, 01.12.2019.

Research output: Contribution to journalArticle

Nishiyama, Keita ; Sugiyama, Makoto ; Yamada, Hiroki ; Makino, Kyoko ; Ishihara, Sayaka ; Takaki, Takashi ; Mukai, Takao ; Okada, Nobuhiko. / A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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