A new hematopoietic cell line, KMT-2, having human interleukin-3 receptors

Sumie Tamura, Minoru Sugawara, Hiroshi Tanaka, Emiko Tezuka, Shin Ichi Nihira, Chikara Miyamoto, Toshio Suda, Yumiko Ohta

Research output: Contribution to journalArticle

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Abstract

A novel human cell line, KMT-2, from umbilical cord blood cells was established based on the selection of cultures in the presence of recombinant human interleukin-3 (IL-3) and the sorting of cells with anti-My 10 antibody. Morphologic and cytochemical studies (peroxidase negative. Sudan-black negative, chloroacetate esterase negative, PAS positive, nonspecific esterase positive) and phenotyping (HLA-DR, My7 = CD13, My9 = CD33, My10 = CD34, MCS-2, LeuM1 positive, glycophorin A negative, and P2 negative) suggest that the KMT-2 cells are myelomonocytic cells, probably of immature progenitor origin. Besides IL-3, granulocyte-macrophage colony-stimulating factor supported the growth of the KMT-2 cells, but IL-1α, IL-2, IL-4, IL-5, and erythropoietin did not. IL-6 showed only slight activity. Binding studies with 125I-labeled recombinant human (rh) IL-3 indicated that IL-3 bound to a single class of high affinity receptors (∼4,000 receptors/cell) on KMT-2 cells with a kd of approximately 200 pmol/L. The chemical cross-linking assay demonstrated that radiolabeled hIL-3 bound three molecules with molecular masses of 170, 130, and 70 Kd. Present data suggest that the newly established human cell line will be a valuable tool for the biologic assay of hIL-3, and a model for biochemical studies of IL-3 receptors.

Original languageEnglish
Pages (from-to)501-507
Number of pages7
JournalBlood
Volume76
Issue number3
Publication statusPublished - 1990 Aug 1

Fingerprint

Interleukin-3 Receptors
Interleukin-3
Cells
Cell Line
Assays
Glycophorin
Carboxylesterase
Interleukin-5
Molecular mass
HLA-DR Antigens
Granulocyte-Macrophage Colony-Stimulating Factor
Erythropoietin
Interleukin-1
Sorting
Cell culture
Interleukin-4
Peroxidase
Interleukin-2
Interleukin-6
Sudan

ASJC Scopus subject areas

  • Hematology

Cite this

Tamura, S., Sugawara, M., Tanaka, H., Tezuka, E., Nihira, S. I., Miyamoto, C., ... Ohta, Y. (1990). A new hematopoietic cell line, KMT-2, having human interleukin-3 receptors. Blood, 76(3), 501-507.

A new hematopoietic cell line, KMT-2, having human interleukin-3 receptors. / Tamura, Sumie; Sugawara, Minoru; Tanaka, Hiroshi; Tezuka, Emiko; Nihira, Shin Ichi; Miyamoto, Chikara; Suda, Toshio; Ohta, Yumiko.

In: Blood, Vol. 76, No. 3, 01.08.1990, p. 501-507.

Research output: Contribution to journalArticle

Tamura, S, Sugawara, M, Tanaka, H, Tezuka, E, Nihira, SI, Miyamoto, C, Suda, T & Ohta, Y 1990, 'A new hematopoietic cell line, KMT-2, having human interleukin-3 receptors', Blood, vol. 76, no. 3, pp. 501-507.
Tamura S, Sugawara M, Tanaka H, Tezuka E, Nihira SI, Miyamoto C et al. A new hematopoietic cell line, KMT-2, having human interleukin-3 receptors. Blood. 1990 Aug 1;76(3):501-507.
Tamura, Sumie ; Sugawara, Minoru ; Tanaka, Hiroshi ; Tezuka, Emiko ; Nihira, Shin Ichi ; Miyamoto, Chikara ; Suda, Toshio ; Ohta, Yumiko. / A new hematopoietic cell line, KMT-2, having human interleukin-3 receptors. In: Blood. 1990 ; Vol. 76, No. 3. pp. 501-507.
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