A New Member of the HCO3- Transporter Superfamily Is an Apical Anion Exchanger of β-Intercalated Cells in the Kidney

Hirohiko Tsuganezawa, Kazuo Kobayashi, Masahiro Iyori, Takashi Araki, Amane Koizumi, Shu Ichi Watanabe, Akimichi Kaneko, Taro Fukao, Toshiaki Monkawa, Tadashi Yoshida, Do Kyong Kim, Yoshikatsu Kanai, Hitoshi Endou, Matsuhiko Hayashi, Takao Saruta

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Abstract

The kidneys play pivotal roles in acid-base homeostasis, and the acid-secreting (α-type) and bicarbonate-secreting (β-type) intercalated cells in the collecting ducts are major sites for the final modulation of urinary acid secretion. Since the H+-ATPase and anion exchanger activities in these two types of intercalated cells exhibit opposite polarities, it has been suggested that the α- and β-intercalated cells are interchangeable via a cell polarity change. Immunohistological studies, however, have failed to confirm that the apical anion exchanger of β-intercalated cells is the band 3 protein localized to the basolateral membrane of α-intercalated cells. In the present study, we show the evidence that a novel member of the anion exchanger and sodium bicarbonate cotransporter superfamily is an apical anion exchanger of β-intercalated cells. Cloned cDNA from the β-intercalated cells shows about 30% homology with anion exchanger types 1-3, and functional expression of this protein in COS-7 cells and Xenopus oocytes showed sodium-independent and 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid-insensitive anion exchanger activity. Furthermore, immunohistological studies revealed that this novel anion exchanger is present on the apical membrane of β-intercalated cells, although some β-intercalated cells were negative for AE4 staining. We conclude that our newly cloned transporter is an apical anion exchanger of the β-intercalated cells, whereas our data do not exclude the possibility that there may be another form of anion exchanger in these cells.

Original languageEnglish
Pages (from-to)8180-8189
Number of pages10
JournalJournal of Biological Chemistry
Volume276
Issue number11
DOIs
Publication statusPublished - 2001 Mar 16

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tsuganezawa, H., Kobayashi, K., Iyori, M., Araki, T., Koizumi, A., Watanabe, S. I., Kaneko, A., Fukao, T., Monkawa, T., Yoshida, T., Kim, D. K., Kanai, Y., Endou, H., Hayashi, M., & Saruta, T. (2001). A New Member of the HCO3- Transporter Superfamily Is an Apical Anion Exchanger of β-Intercalated Cells in the Kidney. Journal of Biological Chemistry, 276(11), 8180-8189. https://doi.org/10.1074/jbc.M004513200