A new predictive factor for hepatocellular carcinoma based on two-dimensional electrophoresis of genomic DNA

Osamu Itano, Masakazu Ueda, Kiyoshi Kikuchi, Motohide Shimazu, Yuko Kitagawa, Kouichi Aiura, Masaki Kitajima

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Molecular genetic analyses have clarified that accumulation of genomic changes provides important steps in carcinogenesis and have identified a number of valuable genetic markers for certain cancers. To date, however, no prognostic markers have been identified for hepatocellular carcinoma (HCC). In this study, we used restriction landmark genomic scanning (RLGS), a new high-speed screening method for multiple genomic changes, to detect unknown genetic alterations in HCC. Thirty-one HCC samples and their normal counterparts were examined by RLGS. Eight spot changes were common in several cases, and all were seen only on the HCC profile. Five of these spots were detected in more than 12 of 31 cases (38.7%). Viral infection had no influence on changes in the RLGS spots. The disease-free survival rate for patients with ≥ 16 changed RLGS spots was significantly lower than that for patients with fewer changed RLGS spots( ≤ 15 spots) (P < 0.001). In multivariate analysis, the number of changed spots was proven to retain an independent prognostic value (relative risk 1.095: P = 0.0031). These results suggest that the number of changed RLGS spots may be a useful biological marker for recurrence of HCC.

Original languageEnglish
Pages (from-to)1676-1683
Number of pages8
JournalOncogene
Volume19
Issue number13
DOIs
Publication statusPublished - 2000 Mar 23

Keywords

  • Disease-free survival
  • Genetic marker
  • HCC
  • Postoperative recurrence
  • RLGS

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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