TY - JOUR
T1 - A new predictive factor for hepatocellular carcinoma based on two-dimensional electrophoresis of genomic DNA
AU - Itano, Osamu
AU - Ueda, Masakazu
AU - Kikuchi, Kiyoshi
AU - Shimazu, Motohide
AU - Kitagawa, Yuko
AU - Aiura, Kouichi
AU - Kitajima, Masaki
N1 - Funding Information:
The authors would like to thank Toshihide Muramatsu, Yuki Inaba and Sachiko Matsuda for their expert technical assistance and Norio Sugimoto ( Sanwa Kagaku Kenkyusho Co. Ltd.) for his valuable advice on statistical analysis. This work was supported by the Grant-in-Aid from the Ministry of Education, Science and Culture and the Ministry of Health and Welfare of Japan and by a National Grant-in-Aid for the Establishment of a High-Tech Research Center in a Private Japanese University.
PY - 2000/3/23
Y1 - 2000/3/23
N2 - Molecular genetic analyses have clarified that accumulation of genomic changes provides important steps in carcinogenesis and have identified a number of valuable genetic markers for certain cancers. To date, however, no prognostic markers have been identified for hepatocellular carcinoma (HCC). In this study, we used restriction landmark genomic scanning (RLGS), a new high-speed screening method for multiple genomic changes, to detect unknown genetic alterations in HCC. Thirty-one HCC samples and their normal counterparts were examined by RLGS. Eight spot changes were common in several cases, and all were seen only on the HCC profile. Five of these spots were detected in more than 12 of 31 cases (38.7%). Viral infection had no influence on changes in the RLGS spots. The disease-free survival rate for patients with ≥ 16 changed RLGS spots was significantly lower than that for patients with fewer changed RLGS spots( ≤ 15 spots) (P < 0.001). In multivariate analysis, the number of changed spots was proven to retain an independent prognostic value (relative risk 1.095: P = 0.0031). These results suggest that the number of changed RLGS spots may be a useful biological marker for recurrence of HCC.
AB - Molecular genetic analyses have clarified that accumulation of genomic changes provides important steps in carcinogenesis and have identified a number of valuable genetic markers for certain cancers. To date, however, no prognostic markers have been identified for hepatocellular carcinoma (HCC). In this study, we used restriction landmark genomic scanning (RLGS), a new high-speed screening method for multiple genomic changes, to detect unknown genetic alterations in HCC. Thirty-one HCC samples and their normal counterparts were examined by RLGS. Eight spot changes were common in several cases, and all were seen only on the HCC profile. Five of these spots were detected in more than 12 of 31 cases (38.7%). Viral infection had no influence on changes in the RLGS spots. The disease-free survival rate for patients with ≥ 16 changed RLGS spots was significantly lower than that for patients with fewer changed RLGS spots( ≤ 15 spots) (P < 0.001). In multivariate analysis, the number of changed spots was proven to retain an independent prognostic value (relative risk 1.095: P = 0.0031). These results suggest that the number of changed RLGS spots may be a useful biological marker for recurrence of HCC.
KW - Disease-free survival
KW - Genetic marker
KW - HCC
KW - Postoperative recurrence
KW - RLGS
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U2 - 10.1038/sj.onc.1203459
DO - 10.1038/sj.onc.1203459
M3 - Article
C2 - 10763824
AN - SCOPUS:0034704794
SN - 0950-9232
VL - 19
SP - 1676
EP - 1683
JO - Oncogene
JF - Oncogene
IS - 13
ER -
