A new rapid protocol for eyeblink conditioning to assess cerebellar motor learning

Kyoichi Emi, Kazuhisa Kohda, Wataru Kakegawa, Sakae Narumi, Michisuke Yuzaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mice with spontaneous and induced mutations causing cerebellar phenotypes have provided key insights into how motor-related memories are stored in cerebellar circuits. Delayed eyeblink conditioning is a form of associative motor learning that depends on the cerebellum. However, neurochemical investigation of the underlying mechanisms has been hampered by the long training period (usually several days) required to establish conditioning. Here, we report a new rapid-training protocol that reliably induced delayed eyeblink conditioning within a single day. The associative memory formation depended on the expression of the δ2 glutamate receptor (GluD2) in cerebellar Purkinje cells. It lasted for several weeks, but could be erased by extinction sessions in a single day. In addition, using the rapid protocol, we found that eyeblink conditioning could be induced in juvenile mice at postnatal day 21, and that the Sindbis-virus-mediated expression of GluD2 could rescue the impaired eyeblink conditioning in GluD2-null mice in vivo.

Original languageEnglish
Pages (from-to)1314-1322
Number of pages9
JournalNeurochemical Research
Volume36
Issue number7
DOIs
Publication statusPublished - 2011 Jul

Fingerprint

Learning
Data storage equipment
Glutamate Receptors
Viruses
Networks (circuits)
Sindbis Virus
Purkinje Cells
Cerebellum
Conditioning (Psychology)
Phenotype
Mutation

Keywords

  • Depression
  • Development
  • Glutamate receptors
  • Long-term
  • Memory
  • Purkinje cells
  • Virus vector

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry

Cite this

A new rapid protocol for eyeblink conditioning to assess cerebellar motor learning. / Emi, Kyoichi; Kohda, Kazuhisa; Kakegawa, Wataru; Narumi, Sakae; Yuzaki, Michisuke.

In: Neurochemical Research, Vol. 36, No. 7, 07.2011, p. 1314-1322.

Research output: Contribution to journalArticle

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