A newly developed immunoliposome - an egg phosphatidylcholine liposome coated with pullulan bearing both a cholesterol moiety and an IgMs fragment

Junzo Sunamoto, Toshinori Sato, Masaki Hirota, Kiyoyasu Fukushima, Kazuhito Hiratani, Kohei Hara

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

An improved methodology for providing a more stable and targetable drug carrier has been developed. This method involves the synthesis of a newly designed immunoliposome by coating the outermost surface of large oligolamellar vesicles of egg phosphatidylcholine with the polysaccharide pullulan, modified to carry both cholesterol, as the hydrophobic anchor, and the monoclonal antibody fragment (anti-sialosyl LewisX, IgMs) as the sensory device. Compared with the binding of pullulan-coated liposomes, that of this immunoliposome to specific cells in vitro was significantly increased by factors of 447 to PC-9 and 295 to KATO-III, but only by a factor of 148 to the less specific cell, 3LL. This strong and specific binding of the immunoliposome to the cell surface of PC-9 was also confirmed by a fluorescence-microscopic investigation using the immunoliposome, which bore the hydrophobic fluorescent probe, terbium trisacetylacetonate, in the liposomal membrane.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalBBA - Biomembranes
Volume898
Issue number3
DOIs
Publication statusPublished - 1987 Apr 23

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Keywords

  • (Human cancer cell line)
  • Cell surface binding
  • Drug carrier
  • Fluorescence
  • Immunoliposome
  • Monoclonal antibody

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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