A novel allelopathic substance, 13-epi-orthosiphol N, in Orthosiphon stamineus

Hisashi Kato-Noguchi, Naoko Hamada, Maho Morita, Kiyotake Suenaga

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Orthosiphon stamineus (Java tea) has been widely used as traditional herb and several bioactive compounds against animal cells have been isolated. However, no bioactive compound against plants has been reported. Therefore, we investigated possible allelopathic properties and substances in O. stamineus. Aqueous methanol extracts of O. stamineus inhibited root and hypocotyl growth of cress (Lepidium sativum) and lettuce (Lactuca sativa) seedlings. Increasing the extract concentration increased the inhibition, which suggests that O. stamineus may have allelopathic properties. When the extract was divided into an ethyl acetate and an aqueous fraction, the ethyl acetate fraction showed the stronger inhibitory effect. Thus, the ethyl acetate phase was further purified, and the main allelopathic substance was isolated and identified as 13- epi-orthosiphol N, a novel compound, by spectral data. 13- epi-Orthosiphol N inhibited root and hypocotyl growth of cress and lettuce at concentrations greater than 10 μmol/L. The concentrations required for 50% inhibition ranged from 41 to 102 μmol/L. These results suggest that 13- epi-orthosiphol N may be an allelochemical and main contributor to the growth inhibitory effect of O. stamineus and may have potential as a template for the development of new plant control substances.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalJournal of Plant Physiology
Volume170
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Orthosiphon
Orthosiphon aristatus
allelochemicals
Lettuce
Brassicaceae
Hypocotyl
lettuce
hypocotyls
extracts
Lepidium sativum
Growth
Plant Development
Pheromones
Lactuca sativa
Seedlings
spectral analysis
Methanol
13-epi-orthosiphol N
herbs
methanol

Keywords

  • Allelopathy
  • Biological activity
  • Growth inhibitor
  • Lamiaceae
  • Orthosiphon stamineus

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

A novel allelopathic substance, 13-epi-orthosiphol N, in Orthosiphon stamineus. / Kato-Noguchi, Hisashi; Hamada, Naoko; Morita, Maho; Suenaga, Kiyotake.

In: Journal of Plant Physiology, Vol. 170, No. 1, 01.01.2013, p. 1-5.

Research output: Contribution to journalArticle

Kato-Noguchi, Hisashi ; Hamada, Naoko ; Morita, Maho ; Suenaga, Kiyotake. / A novel allelopathic substance, 13-epi-orthosiphol N, in Orthosiphon stamineus. In: Journal of Plant Physiology. 2013 ; Vol. 170, No. 1. pp. 1-5.
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