A novel and innovative paper-based analytical device for assessing tear lactoferrin of dry eye patients

Hideki Sonobe; Yoko Ogawa; Kentaro Yamada; Eisuke Shimizu; Yuichi Uchino; Mizuka Kamoi; Yumiko Saijo; Mio Yamane; Daniel Citterio; Koji Suzuki; Kazuo Tsubota

doi:10.1016/j.jtos.2018.11.001

A novel and innovative paper-based analytical device for assessing tear lactoferrin of dry eye patients

Hideki Sonobe, Yoko Ogawa, Kentaro Yamada, Eisuke Shimizu, Yuichi Uchino, Mizuka Kamoi, Yumiko Saijo, Mio Yamane, Daniel Citterio, Koji Suzuki, Kazuo Tsubota

Research output: Contribution to journal › Review article › peer-review

28 Citations (Scopus)

Abstract

Purpose: To elucidate the correlation between lactoferrin concentration in the tear film and signs and symptoms of severe dry eye disease (DED) using a novel microfluidic paper-based analytical device (μPAD) and enzyme-linked immunosorbent assay (ELISA). Methods: Twenty-four patients were recruited at the Keio University Hospital. Using a novel μPAD, lactoferrin concentrations were measured in 4 patients with GVHD-related DED, 3 patients with other types of DED and 2 controls (Group A). For validation by ELISA, 22 patients (7 patients from Group A) comprising 9 patients with GVHD-related DED, 6 patients with other types of DED and 7 controls were examined (Group B). The link between lactoferrin concentration and clinical data about the severity of aqueous tear deficient DED was also investigated by both μPAD and ELISA. Results: The lactoferrin concentration in tear fluid of the DED patients was positively correlated between μPAD and ELISA (p = 0.006, r = 0.886). The tear fluid of the GVHD patients showed low or undetectable lactoferrin concentration. Analysis by ELISA demonstrated that lactoferrin concentrations in the tear film from the GVHD patients were significantly lower than those from the non-GVHD patients (p = 0.010576). ELISA revealed lactoferrin concentration correlated with the value of Schirmer test and tear film breakup time, whereas it was inversely correlated with OSDI, fluorescein and rose bengal scores. Conclusions: The novel μPAD may pave the way for measuring lactoferrin concentration in tear fluid from DED patients. Our results suggested that lactoferrin concentration in tear fluid reflect the severity of DED.

Original language	English
Pages (from-to)	160-166
Number of pages	7
Journal	Ocular Surface
Volume	17
Issue number	1
DOIs	https://doi.org/10.1016/j.jtos.2018.11.001
Publication status	Published - 2019 Jan

Keywords

Dry eye disease
ELISA
Fluorescence detection technique
Lactoferrin
Microfluicic paper-based analytical device

ASJC Scopus subject areas

Ophthalmology

Access to Document

10.1016/j.jtos.2018.11.001

Cite this

@article{91554f6bb2cc4425b228ce5d16b804a0,

title = "A novel and innovative paper-based analytical device for assessing tear lactoferrin of dry eye patients",

abstract = "Purpose: To elucidate the correlation between lactoferrin concentration in the tear film and signs and symptoms of severe dry eye disease (DED) using a novel microfluidic paper-based analytical device (μPAD) and enzyme-linked immunosorbent assay (ELISA). Methods: Twenty-four patients were recruited at the Keio University Hospital. Using a novel μPAD, lactoferrin concentrations were measured in 4 patients with GVHD-related DED, 3 patients with other types of DED and 2 controls (Group A). For validation by ELISA, 22 patients (7 patients from Group A) comprising 9 patients with GVHD-related DED, 6 patients with other types of DED and 7 controls were examined (Group B). The link between lactoferrin concentration and clinical data about the severity of aqueous tear deficient DED was also investigated by both μPAD and ELISA. Results: The lactoferrin concentration in tear fluid of the DED patients was positively correlated between μPAD and ELISA (p = 0.006, r = 0.886). The tear fluid of the GVHD patients showed low or undetectable lactoferrin concentration. Analysis by ELISA demonstrated that lactoferrin concentrations in the tear film from the GVHD patients were significantly lower than those from the non-GVHD patients (p = 0.010576). ELISA revealed lactoferrin concentration correlated with the value of Schirmer test and tear film breakup time, whereas it was inversely correlated with OSDI, fluorescein and rose bengal scores. Conclusions: The novel μPAD may pave the way for measuring lactoferrin concentration in tear fluid from DED patients. Our results suggested that lactoferrin concentration in tear fluid reflect the severity of DED.",

keywords = "Dry eye disease, ELISA, Fluorescence detection technique, Lactoferrin, Microfluicic paper-based analytical device",

author = "Hideki Sonobe and Yoko Ogawa and Kentaro Yamada and Eisuke Shimizu and Yuichi Uchino and Mizuka Kamoi and Yumiko Saijo and Mio Yamane and Daniel Citterio and Koji Suzuki and Kazuo Tsubota",

note = "Funding Information: This work was partially supported by the Japanese Ministry of Education, Science, Sports, Culture and Technology , Japan (# 26462668 and # 18K09421 ) (YO); the Medical Research and Development Programs Focused on Technology Transfer: Development of Advanced Measurement and Analysis Systems (SENTAN) from the Japan Agency for Medical Research and Development (AMED), Japan (DC); and a Research Fellowship of the Japan Society for the Promotion of Science (JSPS) for Young Scientists, Japan (KY). Publisher Copyright: {\textcopyright} 2018 The Authors",

year = "2019",

month = jan,

doi = "10.1016/j.jtos.2018.11.001",

language = "English",

volume = "17",

pages = "160--166",

journal = "Ocular Surface",

issn = "1542-0124",

publisher = "Ethis Communications, Inc.",

number = "1",

}

TY - JOUR

T1 - A novel and innovative paper-based analytical device for assessing tear lactoferrin of dry eye patients

AU - Sonobe, Hideki

AU - Ogawa, Yoko

AU - Yamada, Kentaro

AU - Shimizu, Eisuke

AU - Uchino, Yuichi

AU - Kamoi, Mizuka

AU - Saijo, Yumiko

AU - Yamane, Mio

AU - Citterio, Daniel

AU - Suzuki, Koji

AU - Tsubota, Kazuo

N1 - Funding Information: This work was partially supported by the Japanese Ministry of Education, Science, Sports, Culture and Technology , Japan (# 26462668 and # 18K09421 ) (YO); the Medical Research and Development Programs Focused on Technology Transfer: Development of Advanced Measurement and Analysis Systems (SENTAN) from the Japan Agency for Medical Research and Development (AMED), Japan (DC); and a Research Fellowship of the Japan Society for the Promotion of Science (JSPS) for Young Scientists, Japan (KY). Publisher Copyright: © 2018 The Authors

PY - 2019/1

Y1 - 2019/1

N2 - Purpose: To elucidate the correlation between lactoferrin concentration in the tear film and signs and symptoms of severe dry eye disease (DED) using a novel microfluidic paper-based analytical device (μPAD) and enzyme-linked immunosorbent assay (ELISA). Methods: Twenty-four patients were recruited at the Keio University Hospital. Using a novel μPAD, lactoferrin concentrations were measured in 4 patients with GVHD-related DED, 3 patients with other types of DED and 2 controls (Group A). For validation by ELISA, 22 patients (7 patients from Group A) comprising 9 patients with GVHD-related DED, 6 patients with other types of DED and 7 controls were examined (Group B). The link between lactoferrin concentration and clinical data about the severity of aqueous tear deficient DED was also investigated by both μPAD and ELISA. Results: The lactoferrin concentration in tear fluid of the DED patients was positively correlated between μPAD and ELISA (p = 0.006, r = 0.886). The tear fluid of the GVHD patients showed low or undetectable lactoferrin concentration. Analysis by ELISA demonstrated that lactoferrin concentrations in the tear film from the GVHD patients were significantly lower than those from the non-GVHD patients (p = 0.010576). ELISA revealed lactoferrin concentration correlated with the value of Schirmer test and tear film breakup time, whereas it was inversely correlated with OSDI, fluorescein and rose bengal scores. Conclusions: The novel μPAD may pave the way for measuring lactoferrin concentration in tear fluid from DED patients. Our results suggested that lactoferrin concentration in tear fluid reflect the severity of DED.

AB - Purpose: To elucidate the correlation between lactoferrin concentration in the tear film and signs and symptoms of severe dry eye disease (DED) using a novel microfluidic paper-based analytical device (μPAD) and enzyme-linked immunosorbent assay (ELISA). Methods: Twenty-four patients were recruited at the Keio University Hospital. Using a novel μPAD, lactoferrin concentrations were measured in 4 patients with GVHD-related DED, 3 patients with other types of DED and 2 controls (Group A). For validation by ELISA, 22 patients (7 patients from Group A) comprising 9 patients with GVHD-related DED, 6 patients with other types of DED and 7 controls were examined (Group B). The link between lactoferrin concentration and clinical data about the severity of aqueous tear deficient DED was also investigated by both μPAD and ELISA. Results: The lactoferrin concentration in tear fluid of the DED patients was positively correlated between μPAD and ELISA (p = 0.006, r = 0.886). The tear fluid of the GVHD patients showed low or undetectable lactoferrin concentration. Analysis by ELISA demonstrated that lactoferrin concentrations in the tear film from the GVHD patients were significantly lower than those from the non-GVHD patients (p = 0.010576). ELISA revealed lactoferrin concentration correlated with the value of Schirmer test and tear film breakup time, whereas it was inversely correlated with OSDI, fluorescein and rose bengal scores. Conclusions: The novel μPAD may pave the way for measuring lactoferrin concentration in tear fluid from DED patients. Our results suggested that lactoferrin concentration in tear fluid reflect the severity of DED.

KW - Dry eye disease

KW - ELISA

KW - Fluorescence detection technique

KW - Lactoferrin

KW - Microfluicic paper-based analytical device

UR - http://www.scopus.com/inward/record.url?scp=85057232539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057232539&partnerID=8YFLogxK

U2 - 10.1016/j.jtos.2018.11.001

DO - 10.1016/j.jtos.2018.11.001

M3 - Review article

C2 - 30399438

AN - SCOPUS:85057232539

SN - 1542-0124

VL - 17

SP - 160

EP - 166

JO - Ocular Surface

JF - Ocular Surface

IS - 1

ER -

A novel and innovative paper-based analytical device for assessing tear lactoferrin of dry eye patients

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this