A novel anti-platelet monoclonal antibody induces mouse platelet aggregation through an Fc receptor-independent mechanism

Yukinari Kato, Satoko Hori, Naoya Fujita, Takashi Tsuruo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Platelets are nonproliferative and terminally differentiated cells. Platelets offer an attractive model system to study the various biochemical events leading to structural and functional alterations in activated cells. When platelets are exposed to stimuli, they are activated, undergo a dramatic shape change, adhere to each other, and aggregate. Several monoclonal antibodies (mAbs) that recognize CD9, GPIIb/IIIa (α(IIb)β3 integrins), or GPIV are known to stimulate human platelet aggregation. However, no mAbs able to induce aggregation of mouse platelets have been reported. We have established an anti-mouse platelet mAb (AIP21) that can promote mouse platelet aggregation by itself. Because mouse platelets did not express the Fc receptor (FcR, CD32) on their surfaces and because AIP21 is an IgM subclass, AIP21 might promote platelet aggregation through an FcR-independent mechanism. We could not identify the antigen recognized by AIP21, but flow cytometric analysis revealed that it was not identical to CD9, GPIV, or integrins (i.e., α(IIb), α(v), α5, α6, β1, and β3 integrins). During the aggregation of mouse platelets mediated by AIP2l, several 50-68-kDa proteins are rapidly phosphorylated at tyrosine residues. This phosphorylation by AIP21 was dose-dependent and did not require plasma components. We identified the 52-kDa phosphorylated protein as Shc. These results indicate that AIP21 could be useful for investigating the mechanisms of mouse platelet aggregation.

Original languageEnglish
Pages (from-to)250-255
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume242
Issue number2
DOIs
Publication statusPublished - 1998 Jan 14
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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