A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells

Hidehiko Matsukawa, Takanori Kanai, Makoto Naganuma, Nobuhiko Kamada, Tadakazu Hisamatsu, Hiromasa Takaishi, Haruhiko Ogata, Makio Mukai, Hiromasa Ishii, Tsuneyo Mimori, Mamoru Watanabe, Toshifumi Hibi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. Apoptosis is a crucial element in the behavior of mammalian cells in many different situations. We here report the establishment of a novel monoclonal antibody (anti-LHK mAb) that has apoptosis-inducing activity against colon cancer Colo205 cells. Methods. The mechanism of anti-LHK mAb-induced cell death was assessed by microscopic morphology, Annexin V/Hoechst 33528 staining, and detection of DNA fragmentation. The molecular weight of LHK antigen was determined by Western blotting. Growth inhibition of Colo205 cells induced by anti-LHK mAb was determined by in vitro and in vivo studies. Results. Anti-LHK reacted with a 70-kDa antigen and completely blocked the proliferation of Colo205 cells bearing LHK in vitro in a manner characteristic of apoptosis. Strikingly, anti-LHK mAb suppressed tumor growth in a murine peritoneal dissemination model. Conclusions. LHK antigen, which is restricted to epithelial cells, may be a novel death receptor that plays a critical role in controlling the growth, invasion, and metastasis of human colon cancer cells.

Original languageEnglish
Pages (from-to)945-955
Number of pages11
JournalJournal of gastroenterology
Volume40
Issue number10
DOIs
Publication statusPublished - 2005 Oct 1

Keywords

  • Anti-LHK mAb
  • Apoptosis
  • Colon cancer
  • Fas

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells'. Together they form a unique fingerprint.

  • Cite this

    Matsukawa, H., Kanai, T., Naganuma, M., Kamada, N., Hisamatsu, T., Takaishi, H., Ogata, H., Mukai, M., Ishii, H., Mimori, T., Watanabe, M., & Hibi, T. (2005). A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells. Journal of gastroenterology, 40(10), 945-955. https://doi.org/10.1007/s00535-005-1650-8