A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells

Hidehiko Matsukawa, Takanori Kanai, Makoto Naganuma, Nobuhiko Kamada, Tadakazu Hisamatsu, Hiromasa Takaishi, Haruhiko Ogata, Makio Mukai, Hiromasa Ishii, Tsuneyo Mimori, Mamoru Watanabe, Toshifumi Hibi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. Apoptosis is a crucial element in the behavior of mammalian cells in many different situations. We here report the establishment of a novel monoclonal antibody (anti-LHK mAb) that has apoptosis-inducing activity against colon cancer Colo205 cells. Methods. The mechanism of anti-LHK mAb-induced cell death was assessed by microscopic morphology, Annexin V/Hoechst 33528 staining, and detection of DNA fragmentation. The molecular weight of LHK antigen was determined by Western blotting. Growth inhibition of Colo205 cells induced by anti-LHK mAb was determined by in vitro and in vivo studies. Results. Anti-LHK reacted with a 70-kDa antigen and completely blocked the proliferation of Colo205 cells bearing LHK in vitro in a manner characteristic of apoptosis. Strikingly, anti-LHK mAb suppressed tumor growth in a murine peritoneal dissemination model. Conclusions. LHK antigen, which is restricted to epithelial cells, may be a novel death receptor that plays a critical role in controlling the growth, invasion, and metastasis of human colon cancer cells.

Original languageEnglish
Pages (from-to)945-955
Number of pages11
JournalJournal of Gastroenterology
Volume40
Issue number10
DOIs
Publication statusPublished - 2005 Oct

Fingerprint

Surface Antigens
Colonic Neoplasms
Monoclonal Antibodies
Apoptosis
Antigens
Growth
Death Domain Receptors
Annexin A5
DNA Fragmentation
Cell Death
Molecular Weight
Western Blotting
Epithelial Cells
Cell Proliferation
Staining and Labeling
Neoplasm Metastasis
Neoplasms
In Vitro Techniques

Keywords

  • Anti-LHK mAb
  • Apoptosis
  • Colon cancer
  • Fas

ASJC Scopus subject areas

  • Gastroenterology

Cite this

A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells. / Matsukawa, Hidehiko; Kanai, Takanori; Naganuma, Makoto; Kamada, Nobuhiko; Hisamatsu, Tadakazu; Takaishi, Hiromasa; Ogata, Haruhiko; Mukai, Makio; Ishii, Hiromasa; Mimori, Tsuneyo; Watanabe, Mamoru; Hibi, Toshifumi.

In: Journal of Gastroenterology, Vol. 40, No. 10, 10.2005, p. 945-955.

Research output: Contribution to journalArticle

Matsukawa, Hidehiko ; Kanai, Takanori ; Naganuma, Makoto ; Kamada, Nobuhiko ; Hisamatsu, Tadakazu ; Takaishi, Hiromasa ; Ogata, Haruhiko ; Mukai, Makio ; Ishii, Hiromasa ; Mimori, Tsuneyo ; Watanabe, Mamoru ; Hibi, Toshifumi. / A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells. In: Journal of Gastroenterology. 2005 ; Vol. 40, No. 10. pp. 945-955.
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AU - Watanabe, Mamoru

AU - Hibi, Toshifumi

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AB - Background. Apoptosis is a crucial element in the behavior of mammalian cells in many different situations. We here report the establishment of a novel monoclonal antibody (anti-LHK mAb) that has apoptosis-inducing activity against colon cancer Colo205 cells. Methods. The mechanism of anti-LHK mAb-induced cell death was assessed by microscopic morphology, Annexin V/Hoechst 33528 staining, and detection of DNA fragmentation. The molecular weight of LHK antigen was determined by Western blotting. Growth inhibition of Colo205 cells induced by anti-LHK mAb was determined by in vitro and in vivo studies. Results. Anti-LHK reacted with a 70-kDa antigen and completely blocked the proliferation of Colo205 cells bearing LHK in vitro in a manner characteristic of apoptosis. Strikingly, anti-LHK mAb suppressed tumor growth in a murine peritoneal dissemination model. Conclusions. LHK antigen, which is restricted to epithelial cells, may be a novel death receptor that plays a critical role in controlling the growth, invasion, and metastasis of human colon cancer cells.

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