A novel combination of serum microRNAs for the detection of early gastric cancer

Seiichiro Abe, Juntaro Matsuzaki, Kazuki Sudo, Ichiro Oda, Hitoshi Katai, Ken Kato, Satoko Takizawa, Hiromi Sakamoto, Fumitaka Takeshita, Shumpei Niida, Yutaka Saito, Takahiro Ochiya

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background: The aim of this study was to identify serum miRNAs that discriminate early gastric cancer (EGC) samples from non-cancer controls using a large cohort. Methods: This retrospective case–control study included 1417 serum samples from patients with EGC (seen at the National Cancer Center Hospital in Tokyo between 2008 and 2012) and 1417 age- and gender-matched non-cancer controls. The samples were randomly assigned to discovery and validation sets and the miRNA expression profiles of whole serum samples were comprehensively evaluated using a highly sensitive DNA chip (3D-Gene®) designed to detect 2565 miRNA sequences. Diagnostic models were constructed using the levels of several miRNAs in the discovery set, and the diagnostic performance of the model was evaluated in the validation set. Results: The discovery set consisted of 708 samples from EGC patients and 709 samples from non-cancer controls, and the validation set consisted of 709 samples from EGC patients and 708 samples from non-cancer controls. The diagnostic EGC index was constructed using four miRNAs (miR-4257, miR-6785-5p, miR-187-5p, and miR-5739). In the discovery set, a receiver operating characteristic curve analysis of the EGC index revealed that the area under the curve (AUC) was 0.996 with a sensitivity of 0.983 and a specificity of 0.977. In the validation set, the AUC for the EGC index was 0.998 with a sensitivity of 0.996 and a specificity of 0.953. Conclusions: A novel combination of four serum miRNAs could be a useful non-invasive diagnostic biomarker to detect EGC with high accuracy. A multicenter prospective study is ongoing to confirm the present observations.

Original languageEnglish
Pages (from-to)835-843
Number of pages9
JournalGastric Cancer
Volume24
Issue number4
DOIs
Publication statusPublished - 2021 Jul
Externally publishedYes

Keywords

  • Early gastric cancer
  • Gastric cancer
  • Microarray analysis
  • microRNA
  • Screening

ASJC Scopus subject areas

  • Oncology
  • Gastroenterology
  • Cancer Research

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