TY - JOUR
T1 - A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins
AU - Mitsui, Kaoru
AU - Nakanishi, Makoto
AU - Ohtsuka, Satoshi
AU - Norwood, Thomas H.
AU - Okabayashi, Ken
AU - Miyamoto, Chikara
AU - Tanaka, Keiji
AU - Yoshimura, Akihiko
AU - Ohtsubo, Motoaki
N1 - Funding Information:
We especially thank B. L. Kelly, S. M. Hollenberg, S. J. Elledge, K. Yamashita, N. Hayashi, T. Sekiguchi, and M. Chen for reagents used in the two-hybrid screening, Mitchell L. Martin (Hoffmann–LaRoche Co.) for help in identifying human Ceb1 genomic DNA by bioinfor-matics, D. A. Galloway for the use of the HPV E6 and E7 retrovirus vectors, M. Ouchida for plasmids, and J. M. Roberts and T. Chiba-zakura for materials and critical comments. Part of this work was supported by grants from the Ministry of Science, Education and Culture of Japan, the Naito Memorial Foundation, the Uehara Memorial Foundation, and the Ciba-Geigy Foundation (Japan) for the Promotion of Science. K.T. and M.O. are supported by a Grant-in-Aid for Scientific Research on Priority Areas (Intracellular Proteolysis). O.M. is a special fellow of the Leukemia Society of America.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1999/12/9
Y1 - 1999/12/9
N2 - Cyclin E-Cdk2 is an evolutionary conserved cyclin-dependent kinase (CDK) complex that drives the G1 to S phase transition of the cell cycle. A novel cDNA encoding a HECT family protein also containing RCC1-like repeats was isolated by a yeast two-hybrid screening using both cyclin E and its inhibitor p21. The protein product of this cDNA, Ceb1, interacts with various cyclin subunits of CDKs in mammalian cells. Expression of Ceb1 is specifically detected in testis and ovary and is highly elevated when the functions of the tumor suppressor proteins, p53 and RB, are compromised by mutations or viral oncoproteins. The present results suggest that Ceb1 may play a critical role when its expression and the CDK activity are upregulated by inactivation of p53 and RB.
AB - Cyclin E-Cdk2 is an evolutionary conserved cyclin-dependent kinase (CDK) complex that drives the G1 to S phase transition of the cell cycle. A novel cDNA encoding a HECT family protein also containing RCC1-like repeats was isolated by a yeast two-hybrid screening using both cyclin E and its inhibitor p21. The protein product of this cDNA, Ceb1, interacts with various cyclin subunits of CDKs in mammalian cells. Expression of Ceb1 is specifically detected in testis and ovary and is highly elevated when the functions of the tumor suppressor proteins, p53 and RB, are compromised by mutations or viral oncoproteins. The present results suggest that Ceb1 may play a critical role when its expression and the CDK activity are upregulated by inactivation of p53 and RB.
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U2 - 10.1006/bbrc.1999.1777
DO - 10.1006/bbrc.1999.1777
M3 - Article
C2 - 10581175
AN - SCOPUS:0033539930
SN - 0006-291X
VL - 266
SP - 115
EP - 122
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -