A novel hydroxyapatite fiber mesh as a carrier for recombinant human bone morphogenetic protein-2 enhances bone union in rat posterolateral fusion model

Hikaru Morisue, Morio Matsumoto, Kazuhiro Chiba, Hideo Matsumoto, Yoshiaki Toyama, Mamoru Aizawa, Nobuyuki Kanzawa, Takahiro J. Fujimi, Hiroshi Uchida, Isao Okada

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Study Design. An experimental study, in which spinal fusion in rats was conducted using a hydroxyapatite fiber mesh (HAM) as a carrier for recombinant human bone morphogenetic protein (rhBMP)-2. Objectives. To study the usefulness of the HAM as a carrier and seek the possibility of clinical application in spinal fusion. Summary of Background Data. Several biomaterials have been used as a carrier for BMP to achieve spine fusion, however, to our knowledge, the most effective carrier has not been established. Methods. In experiment No. 1, HAMs and the controls (commercially available hydroxyapatite ceramic body), loaded with rhBMP-2, were immersed in phosphate-buffered saline to evaluate the time course of the release of rhBMP-2. In experiment No. 2, posterolateral fusion was conducted in rats using HAM and the control loaded with rhBMP-2. The fusion status was evaluated radiologically and histologically after surgery. Results. In experiment No. 1, HAMs released a larger amount of rhBMP-2 for up to 28 days than the controls (49.5% vs 7.8%). In experiment No. 2, the fusion rate was significantly higher in the HAM group (>80%) than in the control group (20%). Dense new bone formed close to the spine, and the HAMs were markedly absorbed compared with the controls. Conclusion. HAM provided more solid fusion mass than the control, suggesting that HAM is an efficient carrier for BMP.

Original languageEnglish
Pages (from-to)1194-1200
Number of pages7
JournalSpine
Volume31
Issue number11
DOIs
Publication statusPublished - 2006 May

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Durapatite
Bone and Bones
Spinal Fusion
Spine
Ceramics
Biocompatible Materials
recombinant human bone morphogenetic protein-2
Phosphates
Control Groups

Keywords

  • Bioabsorbability
  • Bone ingrowth
  • Carrier
  • Hydroxyapatite
  • Posterolateral fusion
  • Recombinant human bone morphogenetic protein-2

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Orthopedics and Sports Medicine

Cite this

A novel hydroxyapatite fiber mesh as a carrier for recombinant human bone morphogenetic protein-2 enhances bone union in rat posterolateral fusion model. / Morisue, Hikaru; Matsumoto, Morio; Chiba, Kazuhiro; Matsumoto, Hideo; Toyama, Yoshiaki; Aizawa, Mamoru; Kanzawa, Nobuyuki; Fujimi, Takahiro J.; Uchida, Hiroshi; Okada, Isao.

In: Spine, Vol. 31, No. 11, 05.2006, p. 1194-1200.

Research output: Contribution to journalArticle

Morisue, Hikaru ; Matsumoto, Morio ; Chiba, Kazuhiro ; Matsumoto, Hideo ; Toyama, Yoshiaki ; Aizawa, Mamoru ; Kanzawa, Nobuyuki ; Fujimi, Takahiro J. ; Uchida, Hiroshi ; Okada, Isao. / A novel hydroxyapatite fiber mesh as a carrier for recombinant human bone morphogenetic protein-2 enhances bone union in rat posterolateral fusion model. In: Spine. 2006 ; Vol. 31, No. 11. pp. 1194-1200.
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abstract = "Study Design. An experimental study, in which spinal fusion in rats was conducted using a hydroxyapatite fiber mesh (HAM) as a carrier for recombinant human bone morphogenetic protein (rhBMP)-2. Objectives. To study the usefulness of the HAM as a carrier and seek the possibility of clinical application in spinal fusion. Summary of Background Data. Several biomaterials have been used as a carrier for BMP to achieve spine fusion, however, to our knowledge, the most effective carrier has not been established. Methods. In experiment No. 1, HAMs and the controls (commercially available hydroxyapatite ceramic body), loaded with rhBMP-2, were immersed in phosphate-buffered saline to evaluate the time course of the release of rhBMP-2. In experiment No. 2, posterolateral fusion was conducted in rats using HAM and the control loaded with rhBMP-2. The fusion status was evaluated radiologically and histologically after surgery. Results. In experiment No. 1, HAMs released a larger amount of rhBMP-2 for up to 28 days than the controls (49.5{\%} vs 7.8{\%}). In experiment No. 2, the fusion rate was significantly higher in the HAM group (>80{\%}) than in the control group (20{\%}). Dense new bone formed close to the spine, and the HAMs were markedly absorbed compared with the controls. Conclusion. HAM provided more solid fusion mass than the control, suggesting that HAM is an efficient carrier for BMP.",
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AU - Toyama, Yoshiaki

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AB - Study Design. An experimental study, in which spinal fusion in rats was conducted using a hydroxyapatite fiber mesh (HAM) as a carrier for recombinant human bone morphogenetic protein (rhBMP)-2. Objectives. To study the usefulness of the HAM as a carrier and seek the possibility of clinical application in spinal fusion. Summary of Background Data. Several biomaterials have been used as a carrier for BMP to achieve spine fusion, however, to our knowledge, the most effective carrier has not been established. Methods. In experiment No. 1, HAMs and the controls (commercially available hydroxyapatite ceramic body), loaded with rhBMP-2, were immersed in phosphate-buffered saline to evaluate the time course of the release of rhBMP-2. In experiment No. 2, posterolateral fusion was conducted in rats using HAM and the control loaded with rhBMP-2. The fusion status was evaluated radiologically and histologically after surgery. Results. In experiment No. 1, HAMs released a larger amount of rhBMP-2 for up to 28 days than the controls (49.5% vs 7.8%). In experiment No. 2, the fusion rate was significantly higher in the HAM group (>80%) than in the control group (20%). Dense new bone formed close to the spine, and the HAMs were markedly absorbed compared with the controls. Conclusion. HAM provided more solid fusion mass than the control, suggesting that HAM is an efficient carrier for BMP.

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