Abstract
A novel spectrophotometric nitrite (NO- 2) nitrate (NO- 3) assay system for a small quantity (5 μl) of dialysate sample obtained by in vivo brain microdialysis was developed based on the diazotization reaction. The system has the advantages of in vivo consecutive measurement, high precision, good reproducibility, technical simplicity, relatively short resolution time (2.5-20 min), and wide availability. The NO- 3 level in the rat striatum was found to be 3 times higher than the NO- 2 level. A nitric oxide (NO) synthase inhibitor, NG-nitro-l-arginine methyl ester, reduced striatal NO- 2 citrulline formation in a dose-related manner and increased arginine, indicating that the tissue NO- 2 level detected by this assay system adequately reflects the striatal NO synthase activity.
| Original language | English |
|---|---|
| Pages (from-to) | 165-168 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 176 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1994 Aug 1 |
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Keywords
- Arginine
- Citrulline
- In vivo microdialysis
- N-Nitro-l-arginine methyl ester
- Nitrate
- Nitric oxide
- Nitrite
- Striatum
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique. / Ohta, Kouichi; Araki, Nobuo; Shibata, Mamoru; Hamada, Junichi; Komatsumoto, Satoru; Shimazu, Kunio; Fukuuchi, Yasuo.
In: Neuroscience Letters, Vol. 176, No. 2, 01.08.1994, p. 165-168.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique
AU - Ohta, Kouichi
AU - Araki, Nobuo
AU - Shibata, Mamoru
AU - Hamada, Junichi
AU - Komatsumoto, Satoru
AU - Shimazu, Kunio
AU - Fukuuchi, Yasuo
PY - 1994/8/1
Y1 - 1994/8/1
N2 - A novel spectrophotometric nitrite (NO- 2) nitrate (NO- 3) assay system for a small quantity (5 μl) of dialysate sample obtained by in vivo brain microdialysis was developed based on the diazotization reaction. The system has the advantages of in vivo consecutive measurement, high precision, good reproducibility, technical simplicity, relatively short resolution time (2.5-20 min), and wide availability. The NO- 3 level in the rat striatum was found to be 3 times higher than the NO- 2 level. A nitric oxide (NO) synthase inhibitor, NG-nitro-l-arginine methyl ester, reduced striatal NO- 2 citrulline formation in a dose-related manner and increased arginine, indicating that the tissue NO- 2 level detected by this assay system adequately reflects the striatal NO synthase activity.
AB - A novel spectrophotometric nitrite (NO- 2) nitrate (NO- 3) assay system for a small quantity (5 μl) of dialysate sample obtained by in vivo brain microdialysis was developed based on the diazotization reaction. The system has the advantages of in vivo consecutive measurement, high precision, good reproducibility, technical simplicity, relatively short resolution time (2.5-20 min), and wide availability. The NO- 3 level in the rat striatum was found to be 3 times higher than the NO- 2 level. A nitric oxide (NO) synthase inhibitor, NG-nitro-l-arginine methyl ester, reduced striatal NO- 2 citrulline formation in a dose-related manner and increased arginine, indicating that the tissue NO- 2 level detected by this assay system adequately reflects the striatal NO synthase activity.
KW - Arginine
KW - Citrulline
KW - In vivo microdialysis
KW - N-Nitro-l-arginine methyl ester
KW - Nitrate
KW - Nitric oxide
KW - Nitrite
KW - Striatum
UR - http://www.scopus.com/inward/record.url?scp=0028070054&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028070054&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(94)90073-6
DO - 10.1016/0304-3940(94)90073-6
M3 - Article
C2 - 7530352
AN - SCOPUS:0028070054
VL - 176
SP - 165
EP - 168
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -