A novel in vivo inducible dendritic cell ablation model in mice

Megumi Okuyama, Hisako Kayama, Koji Atarashi, Hiroyuki Saiga, Taishi Kimura, Ari Waisman, Masahiro Yamamoto, Kiyoshi Takeda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Dendritic cells (DCs) are involved in T cell activation via their uptake and presentation of antigens. In vivo function of DCs was analyzed using transgenic mouse models that express diphtheria toxin receptor (DTR) or the diphtheria toxin-A subunit (DTA) under the control of the CD11c/Itgax promoter. However, CD11c+ cells are heterogeneous populations that contain several DC subsets. Thus, the in vivo function of each subset of DCs remains to be elucidated. Here, we describe a new inducible DC ablation model, in which DTR expression is induced under the CD11c/Itgax promoter after Cre-mediated excision of a stop cassette (CD11c-iDTR). Crossing of CD11c-iDTR mice with CAG-Cre transgenic mice, expressing Cre recombinase under control of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid promoter, led to the generation of mice, in which DTR was selectively expressed in CD11c+ cells (iDTRΔ mice). We successfully deleted CD11c+ cells in bone marrow-derived DCs in vitro and splenic CD11c+ cells in vivo after DT treatment in iDTRΔ mice. This mouse strain will be a useful tool for generating mice lacking a specific subset of DCs using a transgenic mouse strain, in which the Cre gene is expressed by a DC subset-specific promoter.

Original languageEnglish
Pages (from-to)559-563
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume397
Issue number3
DOIs
Publication statusPublished - 2010 Jul 1
Externally publishedYes

Keywords

  • Cre recombinase
  • Dendritic cells
  • Diphtheria toxin receptor
  • Immunology

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Okuyama, M., Kayama, H., Atarashi, K., Saiga, H., Kimura, T., Waisman, A., Yamamoto, M., & Takeda, K. (2010). A novel in vivo inducible dendritic cell ablation model in mice. Biochemical and Biophysical Research Communications, 397(3), 559-563. https://doi.org/10.1016/j.bbrc.2010.05.157