Abstract
Dendritic cells (DCs) are involved in T cell activation via their uptake and presentation of antigens. In vivo function of DCs was analyzed using transgenic mouse models that express diphtheria toxin receptor (DTR) or the diphtheria toxin-A subunit (DTA) under the control of the CD11c/Itgax promoter. However, CD11c+ cells are heterogeneous populations that contain several DC subsets. Thus, the in vivo function of each subset of DCs remains to be elucidated. Here, we describe a new inducible DC ablation model, in which DTR expression is induced under the CD11c/Itgax promoter after Cre-mediated excision of a stop cassette (CD11c-iDTR). Crossing of CD11c-iDTR mice with CAG-Cre transgenic mice, expressing Cre recombinase under control of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid promoter, led to the generation of mice, in which DTR was selectively expressed in CD11c+ cells (iDTRΔ mice). We successfully deleted CD11c+ cells in bone marrow-derived DCs in vitro and splenic CD11c+ cells in vivo after DT treatment in iDTRΔ mice. This mouse strain will be a useful tool for generating mice lacking a specific subset of DCs using a transgenic mouse strain, in which the Cre gene is expressed by a DC subset-specific promoter.
| Original language | English |
|---|---|
| Pages (from-to) | 559-563 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 397 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2010 Jul |
| Externally published | Yes |
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Keywords
- Cre recombinase
- Dendritic cells
- Diphtheria toxin receptor
- Immunology
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
A novel in vivo inducible dendritic cell ablation model in mice. / Okuyama, Megumi; Kayama, Hisako; Atarashi, Koji; Saiga, Hiroyuki; Kimura, Taishi; Waisman, Ari; Yamamoto, Masahiro; Takeda, Kiyoshi.
In: Biochemical and Biophysical Research Communications, Vol. 397, No. 3, 07.2010, p. 559-563.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel in vivo inducible dendritic cell ablation model in mice
AU - Okuyama, Megumi
AU - Kayama, Hisako
AU - Atarashi, Koji
AU - Saiga, Hiroyuki
AU - Kimura, Taishi
AU - Waisman, Ari
AU - Yamamoto, Masahiro
AU - Takeda, Kiyoshi
PY - 2010/7
Y1 - 2010/7
N2 - Dendritic cells (DCs) are involved in T cell activation via their uptake and presentation of antigens. In vivo function of DCs was analyzed using transgenic mouse models that express diphtheria toxin receptor (DTR) or the diphtheria toxin-A subunit (DTA) under the control of the CD11c/Itgax promoter. However, CD11c+ cells are heterogeneous populations that contain several DC subsets. Thus, the in vivo function of each subset of DCs remains to be elucidated. Here, we describe a new inducible DC ablation model, in which DTR expression is induced under the CD11c/Itgax promoter after Cre-mediated excision of a stop cassette (CD11c-iDTR). Crossing of CD11c-iDTR mice with CAG-Cre transgenic mice, expressing Cre recombinase under control of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid promoter, led to the generation of mice, in which DTR was selectively expressed in CD11c+ cells (iDTRΔ mice). We successfully deleted CD11c+ cells in bone marrow-derived DCs in vitro and splenic CD11c+ cells in vivo after DT treatment in iDTRΔ mice. This mouse strain will be a useful tool for generating mice lacking a specific subset of DCs using a transgenic mouse strain, in which the Cre gene is expressed by a DC subset-specific promoter.
AB - Dendritic cells (DCs) are involved in T cell activation via their uptake and presentation of antigens. In vivo function of DCs was analyzed using transgenic mouse models that express diphtheria toxin receptor (DTR) or the diphtheria toxin-A subunit (DTA) under the control of the CD11c/Itgax promoter. However, CD11c+ cells are heterogeneous populations that contain several DC subsets. Thus, the in vivo function of each subset of DCs remains to be elucidated. Here, we describe a new inducible DC ablation model, in which DTR expression is induced under the CD11c/Itgax promoter after Cre-mediated excision of a stop cassette (CD11c-iDTR). Crossing of CD11c-iDTR mice with CAG-Cre transgenic mice, expressing Cre recombinase under control of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid promoter, led to the generation of mice, in which DTR was selectively expressed in CD11c+ cells (iDTRΔ mice). We successfully deleted CD11c+ cells in bone marrow-derived DCs in vitro and splenic CD11c+ cells in vivo after DT treatment in iDTRΔ mice. This mouse strain will be a useful tool for generating mice lacking a specific subset of DCs using a transgenic mouse strain, in which the Cre gene is expressed by a DC subset-specific promoter.
KW - Cre recombinase
KW - Dendritic cells
KW - Diphtheria toxin receptor
KW - Immunology
UR - http://www.scopus.com/inward/record.url?scp=77954216198&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954216198&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2010.05.157
DO - 10.1016/j.bbrc.2010.05.157
M3 - Article
C2 - 20617552
AN - SCOPUS:77954216198
VL - 397
SP - 559
EP - 563
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -