A novel marker for Purkinje cells, KIAA0864 protein. An analysis based on a monoclonal antibody HFB-16 in developing human cerebellum

Yasuhiro Nakamura, Munehiko Yamamoto, Eriko Oda, Yonehiro Kanemura, Eri Kodama, Atsuyo Yamamoto, Hideyuki Yamamoto, Kenji Miyado, Hirotaka James Okano, Ryouji Fukagawa, Koichi Higaki, Mami Yamasaki, Hideyuki Okano

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In the search for immunohistochemical markers of the developing human brain, a monoclonal antibody, HFB-16, was raised against homogenates from the cerebrum of a 15-gestational-week-old (GW) human fetus and screened on paraffin-embedded human embryonic brain specimens. This antibody was particularly useful as a marker for Purkinje cells in the developing human cerebellum. Positive immunoreactivities with HFB-16 first appeared in the Purkinje cell layer at 17 GW. From 20 to 24 GW, positive immunoreactivities were found above the lamina dissecans. After 25 GW, dendrites of Purkinje cells were found with the HFB-16 antibody, and the nerve fibers of the Purkinje cells became positive after 35 GW. Neurons in the dentate nucleus and external and internal granular layers reacted negatively to this antibody. After 1 year, when the external granular layer faded out, the dendrites of the Purkinje cells reached the pial surface of the cerebellum, and nerve fibers began to develop in the white matter. This antibody was also useful for characterization of components in heterotopic neurons found in various anomaly syndromes such as trisomy 13. Expressional cloning indicated the antigen against HFB-16 to be humain KIAA0864 protein, which is supposed to be an alternative splicing product of p116Rip, whose function has not yet been elucidated. The antigenicity of the KIAA0864 protein was confirmed using human cDNA of the KIAA0864 protein, a protein expression vector, and an HFB-16 antibody.

Original languageEnglish
Pages (from-to)423-430
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume53
Issue number4
DOIs
Publication statusPublished - 2005 Apr

Keywords

  • Cerebellar development
  • Expressional cloning
  • Immunohistochemistry
  • KIAA0864 protein
  • Monoclonal antibody
  • Purkinje cell

ASJC Scopus subject areas

  • Anatomy
  • Histology

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  • Cite this

    Nakamura, Y., Yamamoto, M., Oda, E., Kanemura, Y., Kodama, E., Yamamoto, A., Yamamoto, H., Miyado, K., Okano, H. J., Fukagawa, R., Higaki, K., Yamasaki, M., & Okano, H. (2005). A novel marker for Purkinje cells, KIAA0864 protein. An analysis based on a monoclonal antibody HFB-16 in developing human cerebellum. Journal of Histochemistry and Cytochemistry, 53(4), 423-430. https://doi.org/10.1369/jhc.4A6499.2005