A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae

Satoshi Yoshida, Jun Imoto, Toshiko Minato, Rie Oouchi, Yoshiaki Kamada, Masaru Tomita, Tomoyoshi Soga, Hiroyuki Yoshimoto

Research output: Contribution to journalArticle

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Abstract

Sulfite (SO2) plays an important role in flavour stability in alcoholic beverages, whereas hydrogen sulfide (H2S) has an undesirable aroma. To discover the cellular processes that control SO2 and H2S production, we screened a library of Saccharomyces cerevisiae deletion mutants. Deletion of 12 genes led to increased H2S productivity. Ten of these genes are known to be involved in sulfur-containing amino acid metabolism, whereas UBI4 functions in the ubiquitin-proteasome system and SKP2 encodes an F-box-containing protein whose function is unknown. We found that the skp2 mutant accumulated H2S and SO2, because the adenosylphophosulfate kinase Met14p is a substrate of SCFSkp2 and more stable in the skp2 mutant than in the wild-type strain. Furthermore, the skp2 mutant grew more slowly than the wild-type strain under nutrient-limited conditions. Metabolome analysis showed that the concentration of intracellular cysteine is lower in the skp2 mutant than in the wild-type strain. The slow growth of the skp2 mutant was due to a lower concentration of intracellular cysteine, because the addition of cysteine suppressed the slow growth. In the skp2 mutant, the cysteine biosynthesis proteins Str2p, Str3p and Str4p are more stable than in the wild-type strain. Moreover, supplementation with methionine, S-adenosylmethionine, S-adenosylhomocysteine and homocysteine also suppressed the slow growth. Overexpression of STR1 or STR4 caused a more severe defect in the skp2 mutant. These results suggest that the balance of methionine and cysteine biosynthesis is important for yeast cell growth. Thus, Skp2p is one of the key components regulating this balance and H2S/SO2 production.

Original languageEnglish
Pages (from-to)109-121
Number of pages13
JournalYeast
Volume28
Issue number2
DOIs
Publication statusPublished - 2011 Feb

Fingerprint

Yeast
Cysteine
Saccharomyces cerevisiae
Biosynthesis
Growth
Genes
Methionine
Proteins
F-Box Proteins
S-Adenosylhomocysteine
Flavors
Hydrogen sulfide
Cell growth
Sulfur Amino Acids
S-Adenosylmethionine
Alcoholic Beverages
Hydrogen Sulfide
Sulfites
Metabolism
Metabolome

Keywords

  • F-box protein
  • Hydrogen sulfide
  • SKP2
  • Sulfite
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Yoshida, S., Imoto, J., Minato, T., Oouchi, R., Kamada, Y., Tomita, M., ... Yoshimoto, H. (2011). A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae. Yeast, 28(2), 109-121. https://doi.org/10.1002/yea.1823

A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae. / Yoshida, Satoshi; Imoto, Jun; Minato, Toshiko; Oouchi, Rie; Kamada, Yoshiaki; Tomita, Masaru; Soga, Tomoyoshi; Yoshimoto, Hiroyuki.

In: Yeast, Vol. 28, No. 2, 02.2011, p. 109-121.

Research output: Contribution to journalArticle

Yoshida, S, Imoto, J, Minato, T, Oouchi, R, Kamada, Y, Tomita, M, Soga, T & Yoshimoto, H 2011, 'A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae', Yeast, vol. 28, no. 2, pp. 109-121. https://doi.org/10.1002/yea.1823
Yoshida S, Imoto J, Minato T, Oouchi R, Kamada Y, Tomita M et al. A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae. Yeast. 2011 Feb;28(2):109-121. https://doi.org/10.1002/yea.1823
Yoshida, Satoshi ; Imoto, Jun ; Minato, Toshiko ; Oouchi, Rie ; Kamada, Yoshiaki ; Tomita, Masaru ; Soga, Tomoyoshi ; Yoshimoto, Hiroyuki. / A novel mechanism regulates H2S and SO2 production in Saccharomyces cerevisiae. In: Yeast. 2011 ; Vol. 28, No. 2. pp. 109-121.
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