A novel monoclonal antibody, KOR-SA3544 which reacts to Philadelphia chromosome-positive acute lymphoblastic leukemia cells with high sensitivity

T. Mori, K. Sugita, T. Suzuki, T. Okazaki, A. Manabe, R. Hosoya, S. Mizutani, A. Kinoshita, S. Nakazawa

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35 Citations (Scopus)

Abstract

A monoclonal antibody which primarily reacted with Philadelphia chromosome (Ph1)-positive ALL cells was produced. The reactivity of a monoclonal antibody, KOR-SA3544 (IgG2a) was evaluated on normal hemopoietic cells, 68 leukemic cell lines and freshly obtained cells from 190 patients with leukemia and lymphoma. In cultured cells, KOR-SA3544 reacted with Ph1-positive ALL cell lines (5/5) and leukemic cell lines with 11q23 translocation (3/11). In lymphoid cells, KOR-SA3544 was reactive with all of Ph1-positive ALL (26/26), a part of common ALL (5/38) and one case of early B precursor leukemia with 11q23 translocation, but not with peripheral lymphocytes. Normal mature granulocytes were also strongly stained. In myeloid leukemias, KOR-SA3544 was positive (16/56) only in patients with acute myeloid leukemia with FAB-M2 and overt leukemia following myelodysplastic syndrome, but neither with other types of myeloid leukemias nor with blast crisis in chronic myelogenous leukemia. KOR-SA3544 recognized a 90 KDa protein on the membrane of a leukemic cell line, KOPN-57bi. In normal bone marrow, CD19+/KOR-SA3544+ cells were not identified, while Ph1-positive ALL cells were strongly positive for both antibodies. KOR-SA3544 is useful not only for making the diagnosis of Ph1-positive ALL but for detection of the minimal residual disease during remission.

Original languageEnglish
Pages (from-to)1233-1239
Number of pages7
JournalLeukemia
Volume9
Issue number7
Publication statusPublished - 1995 Jul

Keywords

  • Acute lymphoblastic leukemia
  • Minimal residual disease
  • Monoclonal antibody
  • Philadelphia chromosome

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Mori, T., Sugita, K., Suzuki, T., Okazaki, T., Manabe, A., Hosoya, R., Mizutani, S., Kinoshita, A., & Nakazawa, S. (1995). A novel monoclonal antibody, KOR-SA3544 which reacts to Philadelphia chromosome-positive acute lymphoblastic leukemia cells with high sensitivity. Leukemia, 9(7), 1233-1239.