TY - JOUR
T1 - A novel myosin-like protein (myocilin) expressed in the connecting cilium of the photoreceptor
T2 - Molecular cloning, tissue expression, and chromosomal mapping
AU - Kubota, Ryo
AU - Noda, Setsuko
AU - Wang, Yimin
AU - Minoshima, Shinsei
AU - Asakawa, Shuichi
AU - Kudoh, Jun
AU - Mashima, Yukihiko
AU - Oguchi, Yoshihisa
AU - Shimizu, Nobuyoshi
N1 - Funding Information:
We thank Ms. Harumi Harigai for her excellent manuscript preparation and Drs. Atsushi Takayanagi and Shuji Yamashita for helpful discussion. Porcine tissue was kindly provided by SLA Research, Inc. This work was supported by a Grant-in-Aid for Scienti®c Research on Priority Areas (Human Genome Program) from the Ministry of Education, Science, Sports and Culture of Japan, and a grant from the Research Committee on Chorioretinal Degenerations, the Ministry of Health and Welfare of Japan.
PY - 1997/5/1
Y1 - 1997/5/1
N2 - We have isolated a human cDNA clone encoding a novel acidic protein of MW 55,000 that we designated 'myocilin' since it has homology to myosin and is localized preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells. The deduced amino acid sequence of human myocilin showed significant homologies with nonmuscle myosin of Dictyostelium discoideum in the N-terminal region and also with olfactomedin of bullfrog in the C-terminal region. Myocilin contained a leucine zipper- like motif similar to that seen in kinectin and other cytoskeletal proteins. These findings suggest that myocilin is a novel cytoskeletal protein involved in the morphogenesis of ciliated neuroepithelium such as photoreceptor cells. The myocilin gene (MYOC) was mapped to human chromosome 1q23-q24 by fluorescence in situ hybridization.
AB - We have isolated a human cDNA clone encoding a novel acidic protein of MW 55,000 that we designated 'myocilin' since it has homology to myosin and is localized preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells. The deduced amino acid sequence of human myocilin showed significant homologies with nonmuscle myosin of Dictyostelium discoideum in the N-terminal region and also with olfactomedin of bullfrog in the C-terminal region. Myocilin contained a leucine zipper- like motif similar to that seen in kinectin and other cytoskeletal proteins. These findings suggest that myocilin is a novel cytoskeletal protein involved in the morphogenesis of ciliated neuroepithelium such as photoreceptor cells. The myocilin gene (MYOC) was mapped to human chromosome 1q23-q24 by fluorescence in situ hybridization.
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U2 - 10.1006/geno.1997.4682
DO - 10.1006/geno.1997.4682
M3 - Article
C2 - 9169133
AN - SCOPUS:0031149050
SN - 0888-7543
VL - 41
SP - 360
EP - 369
JO - Genomics
JF - Genomics
IS - 3
ER -