A novel myosin-like protein (myocilin) expressed in the connecting cilium of the photoreceptor: Molecular cloning, tissue expression, and chromosomal mapping

Ryo Kubota, Setsuko Noda, Yimin Wang, Shinsei Minoshima, Shuichi Asakawa, Jun Kudoh, Yukihiko Mashima, Yoshihisa Oguchi, Nobuyoshi Shimizu

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Abstract

We have isolated a human cDNA clone encoding a novel acidic protein of MW 55,000 that we designated 'myocilin' since it has homology to myosin and is localized preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells. The deduced amino acid sequence of human myocilin showed significant homologies with nonmuscle myosin of Dictyostelium discoideum in the N-terminal region and also with olfactomedin of bullfrog in the C-terminal region. Myocilin contained a leucine zipper- like motif similar to that seen in kinectin and other cytoskeletal proteins. These findings suggest that myocilin is a novel cytoskeletal protein involved in the morphogenesis of ciliated neuroepithelium such as photoreceptor cells. The myocilin gene (MYOC) was mapped to human chromosome 1q23-q24 by fluorescence in situ hybridization.

Original languageEnglish
Pages (from-to)360-369
Number of pages10
JournalGenomics
Volume41
Issue number3
DOIs
Publication statusPublished - 1997 May 1

ASJC Scopus subject areas

  • Genetics

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    Kubota, R., Noda, S., Wang, Y., Minoshima, S., Asakawa, S., Kudoh, J., Mashima, Y., Oguchi, Y., & Shimizu, N. (1997). A novel myosin-like protein (myocilin) expressed in the connecting cilium of the photoreceptor: Molecular cloning, tissue expression, and chromosomal mapping. Genomics, 41(3), 360-369. https://doi.org/10.1006/geno.1997.4682