A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster

Takuya Hashimoto; Junko Hashimoto; Noritaka Kagaya; Takehiro Nishimura; Hikaru Suenaga; Makoto Nishiyama; Tomohisa Kuzuyama; Kazuo Shin-ya

doi:10.1038/s41429-021-00410-9

A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster

Takuya Hashimoto, Junko Hashimoto, Noritaka Kagaya, Takehiro Nishimura, Hikaru Suenaga, Makoto Nishiyama, Tomohisa Kuzuyama, Kazuo Shin-ya

Research output: Contribution to journal › Article › peer-review

Abstract

Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.

Original language	English
Pages (from-to)	354-358
Number of pages	5
Journal	Journal of Antibiotics
Volume	74
Issue number	5
DOIs	https://doi.org/10.1038/s41429-021-00410-9
Publication status	Published - 2021 May
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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Hashimoto, T., Hashimoto, J., Kagaya, N., Nishimura, T., Suenaga, H., Nishiyama, M., Kuzuyama, T., & Shin-ya, K. (2021). A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster. Journal of Antibiotics, 74(5), 354-358. https://doi.org/10.1038/s41429-021-00410-9

Hashimoto, T, Hashimoto, J, Kagaya, N, Nishimura, T, Suenaga, H, Nishiyama, M, Kuzuyama, T & Shin-ya, K 2021, 'A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster', Journal of Antibiotics, vol. 74, no. 5, pp. 354-358. https://doi.org/10.1038/s41429-021-00410-9

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title = "A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster",

abstract = "Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.",

author = "Takuya Hashimoto and Junko Hashimoto and Noritaka Kagaya and Takehiro Nishimura and Hikaru Suenaga and Makoto Nishiyama and Tomohisa Kuzuyama and Kazuo Shin-ya",

note = "Funding Information: Acknowledgements This work was supported by Japan Agency for Medical Research and Development (AMED) under Grant Number JP17ae0101002 for KS and Grant-in-Aid for Young Scientists (20K15410) for TH. Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to the Japan Antibiotics Research Association.",

year = "2021",

month = may,

doi = "10.1038/s41429-021-00410-9",

language = "English",

volume = "74",

pages = "354--358",

journal = "Journal of Antibiotics",

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AU - Hashimoto, Takuya

AU - Hashimoto, Junko

AU - Kagaya, Noritaka

AU - Nishimura, Takehiro

AU - Suenaga, Hikaru

AU - Nishiyama, Makoto

AU - Kuzuyama, Tomohisa

AU - Shin-ya, Kazuo

N1 - Funding Information: Acknowledgements This work was supported by Japan Agency for Medical Research and Development (AMED) under Grant Number JP17ae0101002 for KS and Grant-in-Aid for Young Scientists (20K15410) for TH. Publisher Copyright: © 2021, The Author(s), under exclusive licence to the Japan Antibiotics Research Association.

PY - 2021/5

Y1 - 2021/5

N2 - Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.

AB - Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.

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A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster

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