A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster

Takuya Hashimoto, Junko Hashimoto, Noritaka Kagaya, Takehiro Nishimura, Hikaru Suenaga, Makoto Nishiyama, Tomohisa Kuzuyama, Kazuo Shin-ya

Research output: Contribution to journalArticlepeer-review

Abstract

Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.

Original languageEnglish
Pages (from-to)354-358
Number of pages5
JournalJournal of Antibiotics
Volume74
Issue number5
DOIs
Publication statusPublished - 2021 May
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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