A novel tetracycline-dependent transactivator with E2F4 transcriptional activation domain.

K. Akagi, M. Kanai, Hideyuki Saya, T. Kozu, A. Berns

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A tetracycline-controlled gene expression system provides a powerful tool to dissect the functions of gene products. However, it often appears difficult to establish cell lines or transgenic animals stably expressing tetracycline-dependent transactivators, possibly as a result of toxicity of the transactivator domains used. In order to overcome this problem, we developed a novel tetracycline-dependent transactivator that works efficiently in mammalian cells. This transactivator is a fusion of the tet reverse repressor mutant and the transcriptional activating domain of human E2F4, which is ubiquitously expressed in vivo. We demonstrate here that this tetracycline-regulated gene expression system provides a two log transcriptional activation in mammalian cells as assessed by northern blot and luciferase analyses. Combining this system with green fluorescent protein reporter systems or microarray gene expression profiling will facilitate the study of gene function.

Original languageEnglish
JournalNucleic Acids Research
Volume29
Issue number4
Publication statusPublished - 2001 Feb 15
Externally publishedYes

Fingerprint

Trans-Activators
Tetracycline
Transcriptional Activation
Gene Expression
Genetically Modified Animals
Gene Expression Profiling
Green Fluorescent Proteins
Luciferases
Northern Blotting
Genes
Cell Line

ASJC Scopus subject areas

  • Genetics

Cite this

A novel tetracycline-dependent transactivator with E2F4 transcriptional activation domain. / Akagi, K.; Kanai, M.; Saya, Hideyuki; Kozu, T.; Berns, A.

In: Nucleic Acids Research, Vol. 29, No. 4, 15.02.2001.

Research output: Contribution to journalArticle

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