Abstract
Oligodendrocytes are generated from oligodendrocyte precursor cells (OPCs). Mechanisms of OPC differentiation have been extensively examined with two-dimensional cell culture systems. However, these cellular events may be more accurately represented using a three-dimensional (3D) model. In this study, we report the development of a novel 3D OPC culture system using gels composed of a mixture of collagen and hyaluronan, wherein cultured rat primary OPCs can proliferate and differentiate into oligodendrocytes. Our data show that the gel concentration and cell-seeding density are critical factors for the numbers of OPCs and oligodendrocytes in our 3D culture system. In addition, Notch signaling, which supports cell-to-cell communication, may also be important for OPC function in our system because a Notch inhibitor DAPT suppressed OPC proliferation and differentiation. Taken together, cultured rat OPCs can grow in collagen-/hyaluronan-based gels, and our novel 3D OPC culture system may offer a useful platform for examining the mechanisms of OPC function in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 1078-1085 |
| Number of pages | 8 |
| Journal | Stem Cells and Development |
| Volume | 26 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2017 Jul 15 |
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Keywords
- 3D culture
- astrocyte
- cell-cell interaction
- Notch signaling
- oligodendrocyte precursor cell
ASJC Scopus subject areas
- Hematology
- Developmental Biology
- Cell Biology
Cite this
A Novel Three-Dimensional Culture System for Oligodendrocyte Precursor Cells. / Egawa, Naohiro; Shindo, Akihiro; Liang, Anna C.; Du, Yang; Xing, Changhong; Lo, Evan K.; Itoh, Kanako; Kinoshita, Hisanori; Maki, Takakuni; Takahashi, Ryosuke; Sudo, Ryo; Spector, Myron; Lok, Josephine; Arai, Ken.
In: Stem Cells and Development, Vol. 26, No. 14, 15.07.2017, p. 1078-1085.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Novel Three-Dimensional Culture System for Oligodendrocyte Precursor Cells
AU - Egawa, Naohiro
AU - Shindo, Akihiro
AU - Liang, Anna C.
AU - Du, Yang
AU - Xing, Changhong
AU - Lo, Evan K.
AU - Itoh, Kanako
AU - Kinoshita, Hisanori
AU - Maki, Takakuni
AU - Takahashi, Ryosuke
AU - Sudo, Ryo
AU - Spector, Myron
AU - Lok, Josephine
AU - Arai, Ken
PY - 2017/7/15
Y1 - 2017/7/15
N2 - Oligodendrocytes are generated from oligodendrocyte precursor cells (OPCs). Mechanisms of OPC differentiation have been extensively examined with two-dimensional cell culture systems. However, these cellular events may be more accurately represented using a three-dimensional (3D) model. In this study, we report the development of a novel 3D OPC culture system using gels composed of a mixture of collagen and hyaluronan, wherein cultured rat primary OPCs can proliferate and differentiate into oligodendrocytes. Our data show that the gel concentration and cell-seeding density are critical factors for the numbers of OPCs and oligodendrocytes in our 3D culture system. In addition, Notch signaling, which supports cell-to-cell communication, may also be important for OPC function in our system because a Notch inhibitor DAPT suppressed OPC proliferation and differentiation. Taken together, cultured rat OPCs can grow in collagen-/hyaluronan-based gels, and our novel 3D OPC culture system may offer a useful platform for examining the mechanisms of OPC function in vitro.
AB - Oligodendrocytes are generated from oligodendrocyte precursor cells (OPCs). Mechanisms of OPC differentiation have been extensively examined with two-dimensional cell culture systems. However, these cellular events may be more accurately represented using a three-dimensional (3D) model. In this study, we report the development of a novel 3D OPC culture system using gels composed of a mixture of collagen and hyaluronan, wherein cultured rat primary OPCs can proliferate and differentiate into oligodendrocytes. Our data show that the gel concentration and cell-seeding density are critical factors for the numbers of OPCs and oligodendrocytes in our 3D culture system. In addition, Notch signaling, which supports cell-to-cell communication, may also be important for OPC function in our system because a Notch inhibitor DAPT suppressed OPC proliferation and differentiation. Taken together, cultured rat OPCs can grow in collagen-/hyaluronan-based gels, and our novel 3D OPC culture system may offer a useful platform for examining the mechanisms of OPC function in vitro.
KW - 3D culture
KW - astrocyte
KW - cell-cell interaction
KW - Notch signaling
KW - oligodendrocyte precursor cell
UR - http://www.scopus.com/inward/record.url?scp=85023177543&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85023177543&partnerID=8YFLogxK
U2 - 10.1089/scd.2016.0306
DO - 10.1089/scd.2016.0306
M3 - Article
C2 - 28446066
AN - SCOPUS:85023177543
VL - 26
SP - 1078
EP - 1085
JO - Stem Cells and Development
JF - Stem Cells and Development
SN - 1547-3287
IS - 14
ER -