A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer

Norifumi Kawakami, Tatsuya Ueki, Yusuke Amata, Kan Kanamori, Koichi Matsuo, Kunihiko Gekko, Hitoshi Michibata

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The unusual ascidian ability to accumulate high levels of vanadium ions at concentrations of up to 350 mM, a 107-fold increase over that found in seawater, has been attracting interdisciplinary attention for a century. Accumulated VV is finally reduced to VIII via VIV in ascidian vanadocytes. Reducing agents must therefore participate in the reduction. Previously, we identified a vanadium-binding protein, Vanabin2, in which all 18 cysteines form nine disulfide bonds. Here, we report that Vanabin2 is a novel vanadium reductase because partial cleavage of its disulfide bonds results in the reduction of VV to VIV. We propose that Vanabin2 forms a possible electron transfer cascade from the electron donor, NADPH, via glutathione reductase, glutathione, and Vanabin2 to the acceptor, and vanadium ions conjugated through thiol-disulfide exchange reactions.

Original languageEnglish
Pages (from-to)674-679
Number of pages6
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1794
Issue number4
DOIs
Publication statusPublished - 2009 Apr 1
Externally publishedYes

Fingerprint

Vanadium
Oxidoreductases
Disulfides
Electrons
Urochordata
Ions
Glutathione Reductase
Reducing Agents
Seawater
NADP
Sulfhydryl Compounds
Glutathione
Cysteine
Carrier Proteins

Keywords

  • Ascidian
  • Redox
  • Thiol-disulfide exchange reaction
  • Vanabin2
  • Vanadium

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer. / Kawakami, Norifumi; Ueki, Tatsuya; Amata, Yusuke; Kanamori, Kan; Matsuo, Koichi; Gekko, Kunihiko; Michibata, Hitoshi.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1794, No. 4, 01.04.2009, p. 674-679.

Research output: Contribution to journalArticle

Kawakami, Norifumi ; Ueki, Tatsuya ; Amata, Yusuke ; Kanamori, Kan ; Matsuo, Koichi ; Gekko, Kunihiko ; Michibata, Hitoshi. / A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2009 ; Vol. 1794, No. 4. pp. 674-679.
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