A novel Xenopus laevis SRY-related gene, xSox33

Jun Hagiuda, Yoshiki Hiraoka, Masanori Hasegawa, Motoyuki Ogawa, Sadakazu Aiso

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The sex-determining region Y (SRY) gene and its related Sox genes encode transcriptional regulatory factors. In this study, we isolated and sequenced a novel Sox cDNA from African clawed frog (Xenopus laevis). The Sox gene was named xSox33. xSox33 was revealed to encode 244 amino acids. Reverse transcription-polymerase chain reaction (RT-PCR) showed that xSox33 was expressed at very low levels in some frog tissues including lung, ovary, skeletal muscle, testis, brain and heart. Its embryonic expression was also studied by RT-PCR. After the mid-blastula transition, the zygotic expression was initiated during gastrulation and the level was elevated as the embryogenesis proceeded. Electrophoretic mobility shift assay (EMSA) indicated that a recombinant xSox33 polypeptide was capable of binding to the nucleotide sequence AACAAT.

Original languageEnglish
Pages (from-to)140-145
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1628
Issue number2
DOIs
Publication statusPublished - 2003 Jul 28

Fingerprint

sry Genes
Xenopus laevis
Anura
Reverse Transcription
Genes
Polymerase chain reaction
Transcription
Blastula
Polymerase Chain Reaction
Gastrulation
Electrophoretic Mobility Shift Assay
Embryonic Development
Testis
Ovary
Electrophoretic mobility
Skeletal Muscle
Complementary DNA
Amino Acids
Lung
Peptides

Keywords

  • (Xenopus laevis)
  • DNA binding
  • Embryogenesis
  • HMG box
  • SOX
  • SRY

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

A novel Xenopus laevis SRY-related gene, xSox33. / Hagiuda, Jun; Hiraoka, Yoshiki; Hasegawa, Masanori; Ogawa, Motoyuki; Aiso, Sadakazu.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1628, No. 2, 28.07.2003, p. 140-145.

Research output: Contribution to journalArticle

Hagiuda, Jun ; Hiraoka, Yoshiki ; Hasegawa, Masanori ; Ogawa, Motoyuki ; Aiso, Sadakazu. / A novel Xenopus laevis SRY-related gene, xSox33. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 2003 ; Vol. 1628, No. 2. pp. 140-145.
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