A nuclear localization domain in the hnRNP A1 protein

Haruhiko Siomi, Gideon Dreyfuss

Research output: Contribution to journalArticle

417 Citations (Scopus)

Abstract

The heterogeneous nuclear RNP (hnRNP) A1 protein is one of the major pre- mRNA/mRNA binding proteins in eukaryotic cells and one of the most abundant proteins in the nucleus. It is localized to the nucleoplasm and it also shuttles between the nucleus and the cytoplasm. The amino acid sequence of A1 contains two RNP motif RNA-binding domains (RBDs) at the amino terminus and a glycine-rich domain at the carboxyl terminus. This configuration, designated 2x RBD-Gly, is representative of perhaps the largest family of hnRNP proteins. Unlike most nuclear proteins characterized so far, A1 (and most 2x RBD-Gly proteins) does not contain a recognizable nuclear localization signal (NLS). We have found that a segment of ca. 40 amino acids near the carboxyl end of the protein (designated M9) is necessary and sufficient for nuclear localization; attaching this segment to the bacterial protein β- galactosidase or to pyruvate kinase completely localized these otherwise cytoplasmic proteins to the nucleus. The RBDs and another RNA binding motif found in the glycine-rich domain, the RGG box, are not required for A1 nuclear localization. M9 is a novel type of nuclear localization domain as it does not contain sequences similar to classical basic-type NLS. Interestingly, sequences similar to M9 are found in other nuclear RNA- binding proteins including hnRNP A2.

Original languageEnglish
Pages (from-to)551-560
Number of pages10
JournalJournal of Cell Biology
Volume129
Issue number3
DOIs
Publication statusPublished - 1995 May
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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