A nuclear localization domain in the hnRNP A1 protein

Haruhiko Siomi, G. Dreyfuss

Research output: Contribution to journalArticle

410 Citations (Scopus)

Abstract

The heterogeneous nuclear RNP (hnRNP) A1 protein is one of the major pre- mRNA/mRNA binding proteins in eukaryotic cells and one of the most abundant proteins in the nucleus. It is localized to the nucleoplasm and it also shuttles between the nucleus and the cytoplasm. The amino acid sequence of A1 contains two RNP motif RNA-binding domains (RBDs) at the amino terminus and a glycine-rich domain at the carboxyl terminus. This configuration, designated 2x RBD-Gly, is representative of perhaps the largest family of hnRNP proteins. Unlike most nuclear proteins characterized so far, A1 (and most 2x RBD-Gly proteins) does not contain a recognizable nuclear localization signal (NLS). We have found that a segment of ca. 40 amino acids near the carboxyl end of the protein (designated M9) is necessary and sufficient for nuclear localization; attaching this segment to the bacterial protein β- galactosidase or to pyruvate kinase completely localized these otherwise cytoplasmic proteins to the nucleus. The RBDs and another RNA binding motif found in the glycine-rich domain, the RGG box, are not required for A1 nuclear localization. M9 is a novel type of nuclear localization domain as it does not contain sequences similar to classical basic-type NLS. Interestingly, sequences similar to M9 are found in other nuclear RNA- binding proteins including hnRNP A2.

Original languageEnglish
Pages (from-to)551-560
Number of pages10
JournalJournal of Cell Biology
Volume129
Issue number3
Publication statusPublished - 1995
Externally publishedYes

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Nuclear Proteins
Nuclear Localization Signals
Glycine
Proteins
Galactosidases
Nuclear RNA
Pyruvate Kinase
RNA-Binding Proteins
Bacterial Proteins
RNA Precursors
Eukaryotic Cells
varespladib methyl
Amino Acid Sequence
Carrier Proteins
Cytoplasm
Amino Acids
Messenger RNA
RNA-Binding Motifs

ASJC Scopus subject areas

  • Cell Biology

Cite this

A nuclear localization domain in the hnRNP A1 protein. / Siomi, Haruhiko; Dreyfuss, G.

In: Journal of Cell Biology, Vol. 129, No. 3, 1995, p. 551-560.

Research output: Contribution to journalArticle

Siomi, H & Dreyfuss, G 1995, 'A nuclear localization domain in the hnRNP A1 protein', Journal of Cell Biology, vol. 129, no. 3, pp. 551-560.
Siomi, Haruhiko ; Dreyfuss, G. / A nuclear localization domain in the hnRNP A1 protein. In: Journal of Cell Biology. 1995 ; Vol. 129, No. 3. pp. 551-560.
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