A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies

Eiko Ohuchia; Isao Azumano; Shin'ichi Yoshida; Kazushi Iwata; Yasunori Okada

doi:10.1016/0009-8981(95)06199-1

A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies

Eiko Ohuchia, Isao Azumano, Shin'ichi Yoshida, Kazushi Iwata, Yasunori Okada

Department of Pathology

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 μg/l (1.5 pg/assay) and linearity was obtained between 0.156-10 μg/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 ± 18.8 μg/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera.

Original language	English
Pages (from-to)	181-198
Number of pages	18
Journal	Clinica Chimica Acta
Volume	244
Issue number	2
DOIs	https://doi.org/10.1016/0009-8981(95)06199-1
Publication status	Published - 1996 Jan 31

Keywords

Matrix metalloproteinase 7
Monoclonal antibody
Sandwich enzyme immunoassay
Tissue inhibitor of metalloproteinases

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0009-8981(95)06199-1

Cite this

@article{ee4c0beb81944aa391fdbdb3e1f73e80,

title = "A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies",

abstract = "A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 μg/l (1.5 pg/assay) and linearity was obtained between 0.156-10 μg/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 ± 18.8 μg/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera.",

keywords = "Matrix metalloproteinase 7, Monoclonal antibody, Sandwich enzyme immunoassay, Tissue inhibitor of metalloproteinases",

author = "Eiko Ohuchia and Isao Azumano and Shin'ichi Yoshida and Kazushi Iwata and Yasunori Okada",

year = "1996",

month = jan,

day = "31",

doi = "10.1016/0009-8981(95)06199-1",

language = "English",

volume = "244",

pages = "181--198",

journal = "Clinica Chimica Acta",

issn = "0009-8981",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies

AU - Ohuchia, Eiko

AU - Azumano, Isao

AU - Yoshida, Shin'ichi

AU - Iwata, Kazushi

AU - Okada, Yasunori

PY - 1996/1/31

Y1 - 1996/1/31

N2 - A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 μg/l (1.5 pg/assay) and linearity was obtained between 0.156-10 μg/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 ± 18.8 μg/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera.

AB - A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 μg/l (1.5 pg/assay) and linearity was obtained between 0.156-10 μg/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 ± 18.8 μg/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera.

KW - Matrix metalloproteinase 7

KW - Monoclonal antibody

KW - Sandwich enzyme immunoassay

KW - Tissue inhibitor of metalloproteinases

UR - http://www.scopus.com/inward/record.url?scp=0030021904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030021904&partnerID=8YFLogxK

U2 - 10.1016/0009-8981(95)06199-1

DO - 10.1016/0009-8981(95)06199-1

M3 - Article

C2 - 8714436

AN - SCOPUS:0030021904

SN - 0009-8981

VL - 244

SP - 181

EP - 198

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 2

ER -

A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this