A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies

Ken'ichi Obata, Kazushi Iwata, Yasunori Okada, Yumi Kohrin, Eiko Ohuchi, Shin'ichi Yoshida, Masayuki Shinmei, Taro Hayakawa

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 μ/l and linearity was obtained between 31 and 500 μg/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.

Original languageEnglish
Pages (from-to)59-72
Number of pages14
JournalClinica Chimica Acta
Volume211
Issue number1-2
DOIs
Publication statusPublished - 1992 Oct 15

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Matrix Metalloproteinase 3
Matrix Metalloproteinases
Immunoenzyme Techniques
Monoclonal Antibodies
Enzymes
Assays
Rheumatoid Arthritis
Tissue Inhibitor of Metalloproteinases
Synovial Fluid
Horseradish Peroxidase
Serum
Osteoarthritis
Healthy Volunteers
Fluids

Keywords

  • Monoclonal antibody
  • Osteoarthritis
  • Rheumatoid arthritis
  • Sandwich enzyme immunoassay
  • Stromelysin-1

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies. / Obata, Ken'ichi; Iwata, Kazushi; Okada, Yasunori; Kohrin, Yumi; Ohuchi, Eiko; Yoshida, Shin'ichi; Shinmei, Masayuki; Hayakawa, Taro.

In: Clinica Chimica Acta, Vol. 211, No. 1-2, 15.10.1992, p. 59-72.

Research output: Contribution to journalArticle

Obata, K, Iwata, K, Okada, Y, Kohrin, Y, Ohuchi, E, Yoshida, S, Shinmei, M & Hayakawa, T 1992, 'A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies', Clinica Chimica Acta, vol. 211, no. 1-2, pp. 59-72. https://doi.org/10.1016/0009-8981(92)90105-Y
Obata, Ken'ichi ; Iwata, Kazushi ; Okada, Yasunori ; Kohrin, Yumi ; Ohuchi, Eiko ; Yoshida, Shin'ichi ; Shinmei, Masayuki ; Hayakawa, Taro. / A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies. In: Clinica Chimica Acta. 1992 ; Vol. 211, No. 1-2. pp. 59-72.
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