A portable lipid bilayer system for environmental sensing with a transmembrane protein

Ryuji Kawano, Yutaro Tsuji, Koki Kamiya, Taiga Kodama, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.

Original languageEnglish
Article numbere102427
JournalPloS one
Volume9
Issue number7
DOIs
Publication statusPublished - 2014 Jul 29

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Kawano, R., Tsuji, Y., Kamiya, K., Kodama, T., Osaki, T., Miki, N., & Takeuchi, S. (2014). A portable lipid bilayer system for environmental sensing with a transmembrane protein. PloS one, 9(7), [e102427]. https://doi.org/10.1371/journal.pone.0102427