A portable lipid bilayer system for environmental sensing with a transmembrane protein

Ryuji Kawano, Yutaro Tsuji, Koki Kamiya, Taiga Kodama, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.

Original languageEnglish
Article numbere102427
JournalPLoS One
Volume9
Issue number7
DOIs
Publication statusPublished - 2014 Jul 29

Fingerprint

lipid bilayers
Lipid bilayers
transmembrane proteins
Lipid Bilayers
Hemolysin Proteins
hemolysins
ion channels
Ion Channels
membrane proteins
Contacts (fluid mechanics)
droplets
Membrane Proteins
Proteins
Sensors
methodology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kawano, R., Tsuji, Y., Kamiya, K., Kodama, T., Osaki, T., Miki, N., & Takeuchi, S. (2014). A portable lipid bilayer system for environmental sensing with a transmembrane protein. PLoS One, 9(7), [e102427]. https://doi.org/10.1371/journal.pone.0102427

A portable lipid bilayer system for environmental sensing with a transmembrane protein. / Kawano, Ryuji; Tsuji, Yutaro; Kamiya, Koki; Kodama, Taiga; Osaki, Toshihisa; Miki, Norihisa; Takeuchi, Shoji.

In: PLoS One, Vol. 9, No. 7, e102427, 29.07.2014.

Research output: Contribution to journalArticle

Kawano, R, Tsuji, Y, Kamiya, K, Kodama, T, Osaki, T, Miki, N & Takeuchi, S 2014, 'A portable lipid bilayer system for environmental sensing with a transmembrane protein', PLoS One, vol. 9, no. 7, e102427. https://doi.org/10.1371/journal.pone.0102427
Kawano, Ryuji ; Tsuji, Yutaro ; Kamiya, Koki ; Kodama, Taiga ; Osaki, Toshihisa ; Miki, Norihisa ; Takeuchi, Shoji. / A portable lipid bilayer system for environmental sensing with a transmembrane protein. In: PLoS One. 2014 ; Vol. 9, No. 7.
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