A potential link between transgene silencing and poly(A) tails

Mikiko C. Siomi, Hiroko Tsukumo, Akira Ishizuka, Tomoko Nagami, Haruhiko Siomi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Argonaute proteins function in gene silencing induced by double-stranded RNA (dsRNA) in various organisms. In Drosophila, the Argonaute proteins AGO1 and AGO2 have been implicated in post-transcriptional gene-silencing (PTGS)/RNA interference (RNAi). In this study, we found that AGO1 and AGO2 depletion caused the accumulation of multicopied enhanced green fluorescence protein (EGFP) transgene transcripts in Drosophila S2 cells. Depletion of AGO1, the essential factor for miRNA biogenesis, led to an increased transcriptional rate of the transgenes. In contrast, depletion of AGO2, the essential factor for siRNA-directed RNAi, resulted in EGFP mRNA stabilization with concomitant shortening of the EGFP mRNA poly(A) tail. Our findings suggest that AGO1 and AGO2 mediate multicopied transgene silencing by different mechanisms. Intriguingly, Dicer2 depletion phenocopies AGO2 depletion, with an increase in EGFP protein levels and shortening of the EGFP mRNA poly(A) tail. The possibility that AGO2 and Dicer2 involve, at least in part, poly(A) length maintenance of transgene mRNA suggests a potentially important link between transgene silencing and poly(A) tails.

Original languageEnglish
Pages (from-to)1004-1011
Number of pages8
JournalRNA
Volume11
Issue number7
DOIs
Publication statusPublished - 2005 Jul
Externally publishedYes

Fingerprint

Transgenes
Messenger RNA
Fluorescence
Argonaute Proteins
RNA Interference
Proteins
Drosophila
Poly A
Double-Stranded RNA
Gene Silencing
MicroRNAs
Small Interfering RNA
Maintenance

Keywords

  • Argonautes
  • Drosophila
  • Poly(A)
  • PTGS
  • TGS

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Siomi, M. C., Tsukumo, H., Ishizuka, A., Nagami, T., & Siomi, H. (2005). A potential link between transgene silencing and poly(A) tails. RNA, 11(7), 1004-1011. https://doi.org/10.1261/rna.2280105

A potential link between transgene silencing and poly(A) tails. / Siomi, Mikiko C.; Tsukumo, Hiroko; Ishizuka, Akira; Nagami, Tomoko; Siomi, Haruhiko.

In: RNA, Vol. 11, No. 7, 07.2005, p. 1004-1011.

Research output: Contribution to journalArticle

Siomi, MC, Tsukumo, H, Ishizuka, A, Nagami, T & Siomi, H 2005, 'A potential link between transgene silencing and poly(A) tails', RNA, vol. 11, no. 7, pp. 1004-1011. https://doi.org/10.1261/rna.2280105
Siomi MC, Tsukumo H, Ishizuka A, Nagami T, Siomi H. A potential link between transgene silencing and poly(A) tails. RNA. 2005 Jul;11(7):1004-1011. https://doi.org/10.1261/rna.2280105
Siomi, Mikiko C. ; Tsukumo, Hiroko ; Ishizuka, Akira ; Nagami, Tomoko ; Siomi, Haruhiko. / A potential link between transgene silencing and poly(A) tails. In: RNA. 2005 ; Vol. 11, No. 7. pp. 1004-1011.
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