A potential of an Anti-HTLV-I gp46 neutralizing monoclonal antibody (LAT-27) for passive immunization against both horizontal and mother-to-child vertical infection with human T cell leukemia virus type-I

Hideki Fujii, Mamoru Shimizu, Takuya Miyagi, Marie Kunihiro, Reiko Tanaka, Yoshiaki Takahashi, Yuetsu Tanaka

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Although the number of human T-cell leukemia virus type-I (HTLV-I)-infected individuals in the world has been estimated at over 10 million, no prophylaxis vaccines against HTLV-I infection are available. In this study, we took a new approach for establishing the basis of protective vaccines against HTLV-I. We show here the potential of a passively administered HTLV-I neutralizing monoclonal antibody of rat origin (LAT-27) that recognizes epitopes consisting of the HTLV-I gp46 amino acids 191–196. LAT-27 completely blocked HTLV-I infection in vitro at a minimum concentration of 5 μg/mL. Neonatal rats born to mother rats pre-infused with LAT-27 were shown to have acquired a large quantity of LAT-27, and these newborns showed complete resistance against intraperitoneal infection with HTLV-I. On the other hand, when humanized immunodeficient mice were pre-infused intravenously with humanized LAT-27 (hu-LAT-27), all the mice completely resisted HTLV-I infection. These results indicate that hu-LAT-27 may have a potential for passive immunization against both horizontal and mother-to-child vertical infection with HTLV-I.

Original languageEnglish
Article number41
JournalViruses
Volume8
Issue number2
DOIs
Publication statusPublished - 2016 Feb 3
Externally publishedYes

Keywords

  • Envelope gp46
  • HTLV-I
  • Neutralizing monoclonal antibody
  • NOG mice
  • Passive immunity

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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