A practical device for pinpoint delivery of molecules into multiple neurons in culture

Chikako Hara, Kiyohiko Tateyama, Naoki Akamatsu, Hiroyuki Imabayashi, Koichi Karaki, Nobuo Nomura, Hideyuki Okano, Atsushi Miyawaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have developed a device for pinpoint delivery of chemicals, proteins, and nucleic acids into cultured cells. The principle underlying the technique is the flow of molecules from the culture medium into cells through a rupture in the plasma membrane made by a needle puncture. DNA transfection is achieved by stabbing the needle tip into the nucleus. The CellBee device can be attached to any inverted microscope, and molecular delivery can be coupled with conventional live cell imaging. Because the position of the needle relative to the targeted cultured cells is computer-controlled, efficient delivery of molecules such as rhodamine into as many as 100 HeLa cells can be completed in 10 min. Moreover, specific target cells within a single dish can be transfected with multiple DNA constructs by simple changes of culture medium containing different plasmids. In addition, the nano-sized needle tip enables gentle molecular delivery, minimizing cell damage. This method permits DNA transfection into specific hippocampal neurons without disturbing neuronal circuitry established in culture.

Original languageEnglish
Pages (from-to)229-237
Number of pages9
JournalBrain Cell Biology
Volume35
Issue number4-6
DOIs
Publication statusPublished - 2006 Dec

Fingerprint

Needles
Neurons
Equipment and Supplies
Transfection
Culture Media
Cultured Cells
DNA
Rhodamines
HeLa Cells
Punctures
Nucleic Acids
Rupture
Plasmids
Cell Membrane
Proteins

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Hara, C., Tateyama, K., Akamatsu, N., Imabayashi, H., Karaki, K., Nomura, N., ... Miyawaki, A. (2006). A practical device for pinpoint delivery of molecules into multiple neurons in culture. Brain Cell Biology, 35(4-6), 229-237. https://doi.org/10.1007/s11068-008-9021-z

A practical device for pinpoint delivery of molecules into multiple neurons in culture. / Hara, Chikako; Tateyama, Kiyohiko; Akamatsu, Naoki; Imabayashi, Hiroyuki; Karaki, Koichi; Nomura, Nobuo; Okano, Hideyuki; Miyawaki, Atsushi.

In: Brain Cell Biology, Vol. 35, No. 4-6, 12.2006, p. 229-237.

Research output: Contribution to journalArticle

Hara, C, Tateyama, K, Akamatsu, N, Imabayashi, H, Karaki, K, Nomura, N, Okano, H & Miyawaki, A 2006, 'A practical device for pinpoint delivery of molecules into multiple neurons in culture', Brain Cell Biology, vol. 35, no. 4-6, pp. 229-237. https://doi.org/10.1007/s11068-008-9021-z
Hara C, Tateyama K, Akamatsu N, Imabayashi H, Karaki K, Nomura N et al. A practical device for pinpoint delivery of molecules into multiple neurons in culture. Brain Cell Biology. 2006 Dec;35(4-6):229-237. https://doi.org/10.1007/s11068-008-9021-z
Hara, Chikako ; Tateyama, Kiyohiko ; Akamatsu, Naoki ; Imabayashi, Hiroyuki ; Karaki, Koichi ; Nomura, Nobuo ; Okano, Hideyuki ; Miyawaki, Atsushi. / A practical device for pinpoint delivery of molecules into multiple neurons in culture. In: Brain Cell Biology. 2006 ; Vol. 35, No. 4-6. pp. 229-237.
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