A rapid and reliable solid-phase extraction method for high-performance liquid chromatographic analysis of opium alkaloids from Papaver plants

Kayo Yoshimatsu, Fumiyuki Kiuchi, Koichiro Shimomura, Yukiko Makino

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A rapid and reliable solid-phase extraction method for HPLC analysis of opium alkaloids from Papaver plants was established. Fifty mg of dried and powdered plant sample was extracted with 5 ml of 5% acetic acid for 30 min under sonication. After centrifugation, 3 ml of the supernatant was loaded on a reversed-phase cation-exchange solid-phase extraction cartridge. After seriate washings with 0.1 M hydrochloric acid and methanol, alkaloids were eluted with a mixture of 28% ammonia and methanol (1:19). The eluate was concentrated under nitrogen stream at 40°C and the residue was dissolved in 50% aqueous methanol for high performance liquid chromatographic analysis. With this solid-phase extraction method, the recovery of morphine, codeine, oripavine, thebaine, papaverine, noscapine and sanguinarine was from 99.94 to 112.18% when the standard alkaloids were added to the plant samples. Opium alkaloids of a variety of genus Papaver plants cultivated in a field and phytotron were analyzed by this method.

Original languageEnglish
Pages (from-to)1446-1450
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Issue number11
Publication statusPublished - 2005 Nov 1



  • Genus papaver
  • High performance liquid chromatographic analysis
  • Opium alkaloid
  • Solid-phase extraction

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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