A rapid and reliable solid-phase extraction method for high-performance liquid chromatographic analysis of opium alkaloids from Papaver plants

Kayo Yoshimatsu, Fumiyuki Kiuchi, Koichiro Shimomura, Yukiko Makino

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A rapid and reliable solid-phase extraction method for HPLC analysis of opium alkaloids from Papaver plants was established. Fifty mg of dried and powdered plant sample was extracted with 5 ml of 5% acetic acid for 30 min under sonication. After centrifugation, 3 ml of the supernatant was loaded on a reversed-phase cation-exchange solid-phase extraction cartridge. After seriate washings with 0.1 M hydrochloric acid and methanol, alkaloids were eluted with a mixture of 28% ammonia and methanol (1:19). The eluate was concentrated under nitrogen stream at 40°C and the residue was dissolved in 50% aqueous methanol for high performance liquid chromatographic analysis. With this solid-phase extraction method, the recovery of morphine, codeine, oripavine, thebaine, papaverine, noscapine and sanguinarine was from 99.94 to 112.18% when the standard alkaloids were added to the plant samples. Opium alkaloids of a variety of genus Papaver plants cultivated in a field and phytotron were analyzed by this method.

Original languageEnglish
Pages (from-to)1446-1450
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume53
Issue number11
DOIs
Publication statusPublished - 2005 Nov
Externally publishedYes

Fingerprint

Papaver
Opium
Solid Phase Extraction
High performance liquid chromatography
Alkaloids
High Pressure Liquid Chromatography
Methanol
Thebaine
Noscapine
Codeine
Papaverine
Sonication
Centrifugation
Hydrochloric Acid
Ammonia
Washing
Acetic Acid
Morphine
Cations
Ion exchange

Keywords

  • Genus papaver
  • High performance liquid chromatographic analysis
  • Opium alkaloid
  • Solid-phase extraction

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Pharmacology

Cite this

A rapid and reliable solid-phase extraction method for high-performance liquid chromatographic analysis of opium alkaloids from Papaver plants. / Yoshimatsu, Kayo; Kiuchi, Fumiyuki; Shimomura, Koichiro; Makino, Yukiko.

In: Chemical and Pharmaceutical Bulletin, Vol. 53, No. 11, 11.2005, p. 1446-1450.

Research output: Contribution to journalArticle

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