A rapid cross-sectioning and freeze-clamping device for the beating canine heart

Shingo Hori, Hiroe Nakazawa, Yozo Ohnishi, Hideaki Yoshino, Akira Murayama, Yasuhiro Nishikawa, Yoshiro Nakamura, Muneyuki Horikawa, Tadao Hoshino, Motoaki Bessho

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A new sampling method of cross-sectioning the canine heart in situ was developed. A mechanical device, driven by spring power, enabled cross-sectioning of a short-axis plane of the beating canine heart (4 mm thick) with high speed rotating blades, at a pre-determined phase of the cardiac cycle, and instantaneous freeze-clamping (2.4 mm thick) with pre-cooled aluminum blocks, all within 120 ms. By this method, the anatomical structures of the sample were well preserved. Transmural metabolism and flow distribution were instantaneously fixed and high resolution of the two-dimensional redox state was obtained by application of NADH fluorescence photography. Micro-samplings from the desired portion of the cross-sectional slice were possible at -190°C. NADH fluorescence of the samples did not increase from the surface to 1.2 mm in depth, confirming that there was no ischemic artifact. With the present technique, a heart sample in which transmural metabolism, and the redox state, are fixed and visualized is attainable, thus providing a new tool for the study of myocardial ischemia.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume21
Issue number2
DOIs
Publication statusPublished - 1989 Feb
Externally publishedYes

Keywords

  • Adenine nucleotides
  • Creatine phosphate
  • Myocardial biopsy
  • Myocardial ischemia
  • NADH fluorescence
  • Redox state

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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    Hori, S., Nakazawa, H., Ohnishi, Y., Yoshino, H., Murayama, A., Nishikawa, Y., Nakamura, Y., Horikawa, M., Hoshino, T., & Bessho, M. (1989). A rapid cross-sectioning and freeze-clamping device for the beating canine heart. Journal of Molecular and Cellular Cardiology, 21(2), 203-210. https://doi.org/10.1016/0022-2828(89)90862-6