TY - JOUR
T1 - A rapid cross-sectioning and freeze-clamping device for the beating canine heart
AU - Hori, Shingo
AU - Nakazawa, Hiroe
AU - Ohnishi, Yozo
AU - Yoshino, Hideaki
AU - Murayama, Akira
AU - Nishikawa, Yasuhiro
AU - Nakamura, Yoshiro
AU - Horikawa, Muneyuki
AU - Hoshino, Tadao
AU - Bessho, Motoaki
N1 - Funding Information:
Acknowledgements We are grateful to Dr Kyuya Kogure of th(e Tohoku University, and Dr Ren Nishimoto of the Tokyo Institute of Technology for technical advice and encouragement, and to Kazuo Yamaguchi of the Nihon Koden Co., Ltd. for the design and development of the control unit for the sampling device. This study was supported by Grants-in-Aid 58870059,59376027, and 60304061 for Scientific Research from the Ministry of Education, Science and Culture, Japan, and by a Research Grant for 1983 from The Japan Heart Foundation.
PY - 1989/2
Y1 - 1989/2
N2 - A new sampling method of cross-sectioning the canine heart in situ was developed. A mechanical device, driven by spring power, enabled cross-sectioning of a short-axis plane of the beating canine heart (4 mm thick) with high speed rotating blades, at a pre-determined phase of the cardiac cycle, and instantaneous freeze-clamping (2.4 mm thick) with pre-cooled aluminum blocks, all within 120 ms. By this method, the anatomical structures of the sample were well preserved. Transmural metabolism and flow distribution were instantaneously fixed and high resolution of the two-dimensional redox state was obtained by application of NADH fluorescence photography. Micro-samplings from the desired portion of the cross-sectional slice were possible at -190°C. NADH fluorescence of the samples did not increase from the surface to 1.2 mm in depth, confirming that there was no ischemic artifact. With the present technique, a heart sample in which transmural metabolism, and the redox state, are fixed and visualized is attainable, thus providing a new tool for the study of myocardial ischemia.
AB - A new sampling method of cross-sectioning the canine heart in situ was developed. A mechanical device, driven by spring power, enabled cross-sectioning of a short-axis plane of the beating canine heart (4 mm thick) with high speed rotating blades, at a pre-determined phase of the cardiac cycle, and instantaneous freeze-clamping (2.4 mm thick) with pre-cooled aluminum blocks, all within 120 ms. By this method, the anatomical structures of the sample were well preserved. Transmural metabolism and flow distribution were instantaneously fixed and high resolution of the two-dimensional redox state was obtained by application of NADH fluorescence photography. Micro-samplings from the desired portion of the cross-sectional slice were possible at -190°C. NADH fluorescence of the samples did not increase from the surface to 1.2 mm in depth, confirming that there was no ischemic artifact. With the present technique, a heart sample in which transmural metabolism, and the redox state, are fixed and visualized is attainable, thus providing a new tool for the study of myocardial ischemia.
KW - Adenine nucleotides
KW - Creatine phosphate
KW - Myocardial biopsy
KW - Myocardial ischemia
KW - NADH fluorescence
KW - Redox state
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U2 - 10.1016/0022-2828(89)90862-6
DO - 10.1016/0022-2828(89)90862-6
M3 - Article
C2 - 2664189
AN - SCOPUS:0024539931
SN - 0022-2828
VL - 21
SP - 203
EP - 210
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
IS - 2
ER -