A recombinant murine granulocyte/macrophage (GM) colony-stimulating factor derived from an inducer T cell line (IH5.5). Functional restriction to GM progenitor cells

S. Kajigaya, T. Suda, J. Suda, M. Saito, Y. Miura, M. Iizuka, S. Kobayashi, N. Minato, T. Sudo

Research output: Contribution to journalArticle

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Abstract

The cDNA for the murine granulocyte/macrophage colony-stimulating factor (GM-CSF) was cloned from a cDNA library obtained from a murine T cell line, IH5.5, by using two synthetic probes that encoded two parts of the GM-CSF from murine lung. The cDNA inserted into the plasmid vector pcDV1 was transfected into monkey COS-1 cells and the conditioned medium was used to investigate the hemopoietic activities of the resultant product, recombinant GM-CSF (rGM-CSF), by means of various colony assays. rGM-CSF stimulated only neutrophil/macrophage colonies in the cultures of murine normal bone marrow and fetal liver cells. No other colony stimulating activities (CSA) were seen in the preparation including burst-promoting activity, eosinophil-CSA, megakaryocyte-CSA and mast cell-CSA. rGM-CSF could not support colony formation of 5-fluorouracil-treated mouse spleen cells, in which only the primitive population of stem cells survived. However, after culture of these cells with PWM-spleen cell-conditioned medium (PWM-SCM), the colonies consisting of blast cells were formed. These blast cells could now be induced to form neutrophil/macrophage colonies in the presence of rGM-CSF. Pure neutrophil colonies, pure macrophage colonies, as well as mixed neutrophil/macrophage colonies, were formed from these single blast cells in the presence of rGM-CSF by micromanipulation. rGM-CSF did not act on pluripotent hemopoietic stem cells, but did act directly and selectively on neutrophil/macrophage progenitors. Moreover, striking heterogeneities were noted in the size of the colonies and the proportion of components. GM-CSF is, therefore, considered to play a noninstructive role in the differentiation of the GM pathway.

Original languageEnglish
Pages (from-to)1102-1113
Number of pages12
JournalJournal of Experimental Medicine
Volume164
Issue number4
Publication statusPublished - 1986

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Granulocyte-Macrophage Progenitor Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Helper-Inducer T-Lymphocytes
Macrophages
T-Lymphocytes
Cell Line
Neutrophils
Granulocytes
Conditioned Culture Medium
Spleen
Complementary DNA
Micromanipulation
Pluripotent Stem Cells
Megakaryocytes
COS Cells
Gene Library
Eosinophils
Mast Cells
Fluorouracil
Haplorhini

ASJC Scopus subject areas

  • Immunology

Cite this

A recombinant murine granulocyte/macrophage (GM) colony-stimulating factor derived from an inducer T cell line (IH5.5). Functional restriction to GM progenitor cells. / Kajigaya, S.; Suda, T.; Suda, J.; Saito, M.; Miura, Y.; Iizuka, M.; Kobayashi, S.; Minato, N.; Sudo, T.

In: Journal of Experimental Medicine, Vol. 164, No. 4, 1986, p. 1102-1113.

Research output: Contribution to journalArticle

Kajigaya, S, Suda, T, Suda, J, Saito, M, Miura, Y, Iizuka, M, Kobayashi, S, Minato, N & Sudo, T 1986, 'A recombinant murine granulocyte/macrophage (GM) colony-stimulating factor derived from an inducer T cell line (IH5.5). Functional restriction to GM progenitor cells', Journal of Experimental Medicine, vol. 164, no. 4, pp. 1102-1113.
Kajigaya, S. ; Suda, T. ; Suda, J. ; Saito, M. ; Miura, Y. ; Iizuka, M. ; Kobayashi, S. ; Minato, N. ; Sudo, T. / A recombinant murine granulocyte/macrophage (GM) colony-stimulating factor derived from an inducer T cell line (IH5.5). Functional restriction to GM progenitor cells. In: Journal of Experimental Medicine. 1986 ; Vol. 164, No. 4. pp. 1102-1113.
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AU - Saito, M.

AU - Miura, Y.

AU - Iizuka, M.

AU - Kobayashi, S.

AU - Minato, N.

AU - Sudo, T.

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