A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila

Kuniaki Saito, Sachi Inagaki, Toutai Mituyama, Yoshinori Kawamura, Yukiteru Ono, Eri Sakota, Hazuki Kotani, Kiyoshi Asai, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

256 Citations (Scopus)

Abstract

PIWI-interacting RNAs (piRNAs) silence retrotransposons in Drosophila germ lines by associating with the PIWI proteins Argonaute 3 (AGO3), Aubergine (Aub) and Piwi. piRNAs in Drosophila are produced from intergenic repetitive genes and piRNA clusters by two systems: the primary processing pathway and the amplification loop. The amplification loop occurs in a Dicer-independent, PIWI-Slicer-dependent manner. However, primary piRNA processing remains elusive. Here we analysed piRNA processing in a Drosophila ovarian somatic cell line where Piwi, but not Aub or AGO3, is expressed; thus, only the primary piRNAs exist. In addition to flamenco, a Piwi-specific piRNA cluster, traffic jam (tj), a large Maf gene, was determined as a new piRNA cluster. piRNAs arising from tj correspond to the untranslated regions of tj messenger RNA and are sense-oriented. piRNA loading on to Piwi may occur in the cytoplasm. zucchini, a gene encoding a putative cytoplasmic nuclease, is required for tj-derived piRNA production. In tj and piwi mutant ovaries, somatic cells fail to intermingle with germ cells and Fasciclin III is overexpressed. Loss of tj abolishes Piwi expression in gonadal somatic cells. Thus, in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing.

Original languageEnglish
Pages (from-to)1296-1299
Number of pages4
JournalNature
Volume461
Issue number7268
DOIs
Publication statusPublished - 2009 Oct 29

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Small Interfering RNA
Drosophila
RNA
Genes
Solanum melongena
Germ Cells
Argonaute Proteins
Untranslated Regions
Retroelements
Multigene Family
Ovary
Cytoplasm
Cell Line
Messenger RNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., ... Siomi, M. C. (2009). A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. Nature, 461(7268), 1296-1299. https://doi.org/10.1038/nature08501

A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. / Saito, Kuniaki; Inagaki, Sachi; Mituyama, Toutai; Kawamura, Yoshinori; Ono, Yukiteru; Sakota, Eri; Kotani, Hazuki; Asai, Kiyoshi; Siomi, Haruhiko; Siomi, Mikiko C.

In: Nature, Vol. 461, No. 7268, 29.10.2009, p. 1296-1299.

Research output: Contribution to journalArticle

Saito, K, Inagaki, S, Mituyama, T, Kawamura, Y, Ono, Y, Sakota, E, Kotani, H, Asai, K, Siomi, H & Siomi, MC 2009, 'A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila', Nature, vol. 461, no. 7268, pp. 1296-1299. https://doi.org/10.1038/nature08501
Saito K, Inagaki S, Mituyama T, Kawamura Y, Ono Y, Sakota E et al. A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. Nature. 2009 Oct 29;461(7268):1296-1299. https://doi.org/10.1038/nature08501
Saito, Kuniaki ; Inagaki, Sachi ; Mituyama, Toutai ; Kawamura, Yoshinori ; Ono, Yukiteru ; Sakota, Eri ; Kotani, Hazuki ; Asai, Kiyoshi ; Siomi, Haruhiko ; Siomi, Mikiko C. / A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. In: Nature. 2009 ; Vol. 461, No. 7268. pp. 1296-1299.
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