A role for borg5 during trophectoderm differentiation.

Queenie P. Vong, Zhonghua Liu, Jae Gyu Yoo, Rong Chen, Wen Xie, Alexei A. Sharov, Chen Ming Fan, Chengyu Liu, Minoru Ko, Yixian Zheng

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Stem cell differentiation is accompanied by a gradual cellular morphogenesis and transcriptional changes. Identification of morphological regulators that control cell behavior during differentiation could shed light on how cell morphogenesis is coupled to transcriptional changes during development. By analyzing cellular behavior during differentiation of mouse embryonic stem cells (ESCs), we uncover a role of Borg5 (binder of Rho guanosine 5'-triphosphatase 5) in regulating trophectoderm (TE) cell morphogenesis. We report that differentiation of ESCs toward TE is accompanied by enhanced actin protrusion and cell motility that require upregulation of Borg5. Borg5 interacts with both Cdc42 and atypical protein kinase C (aPKC) and functions downstream of Cdc42 to enhance TE cell motility. Borg5 is required for the sorting of differentiating TE to the outside of ESCs in vitro. In developing embryos, Borg5 protein localizes to cell-cell contacts and the cytoplasm after compaction. It exhibits higher levels of expression in outer cells than in inner cells in morula and blastocysts. Reduction of Borg5 disrupts aPKC localization and inhibits blastocyst formation. Since Cdx2 and Borg5 facilitate each other's expression as ESCs differentiate toward TE, we propose that cell morphogenesis is coupled with transcriptional changes to regulate TE differentiation. Our studies also demonstrate the utility of ESCs in identifying morphological regulators important for development.

Original languageEnglish
Pages (from-to)1030-1038
Number of pages9
JournalStem cells (Dayton, Ohio)
Volume28
Issue number6
DOIs
Publication statusPublished - 2010 Jun
Externally publishedYes

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Guanosine
Embryonic Stem Cells
Morphogenesis
Blastocyst
Cell Movement
cdc42 GTP-Binding Protein
Morula
Behavior Control
Actins
Cell Differentiation
Cytoplasm
Up-Regulation
Stem Cells
Embryonic Structures
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Vong, Q. P., Liu, Z., Yoo, J. G., Chen, R., Xie, W., Sharov, A. A., ... Zheng, Y. (2010). A role for borg5 during trophectoderm differentiation. Stem cells (Dayton, Ohio), 28(6), 1030-1038. https://doi.org/10.1002/stem.428

A role for borg5 during trophectoderm differentiation. / Vong, Queenie P.; Liu, Zhonghua; Yoo, Jae Gyu; Chen, Rong; Xie, Wen; Sharov, Alexei A.; Fan, Chen Ming; Liu, Chengyu; Ko, Minoru; Zheng, Yixian.

In: Stem cells (Dayton, Ohio), Vol. 28, No. 6, 06.2010, p. 1030-1038.

Research output: Contribution to journalArticle

Vong, QP, Liu, Z, Yoo, JG, Chen, R, Xie, W, Sharov, AA, Fan, CM, Liu, C, Ko, M & Zheng, Y 2010, 'A role for borg5 during trophectoderm differentiation.', Stem cells (Dayton, Ohio), vol. 28, no. 6, pp. 1030-1038. https://doi.org/10.1002/stem.428
Vong QP, Liu Z, Yoo JG, Chen R, Xie W, Sharov AA et al. A role for borg5 during trophectoderm differentiation. Stem cells (Dayton, Ohio). 2010 Jun;28(6):1030-1038. https://doi.org/10.1002/stem.428
Vong, Queenie P. ; Liu, Zhonghua ; Yoo, Jae Gyu ; Chen, Rong ; Xie, Wen ; Sharov, Alexei A. ; Fan, Chen Ming ; Liu, Chengyu ; Ko, Minoru ; Zheng, Yixian. / A role for borg5 during trophectoderm differentiation. In: Stem cells (Dayton, Ohio). 2010 ; Vol. 28, No. 6. pp. 1030-1038.
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