A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor.

Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, Carl P. Blobel

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

All ligands of the epidermal growth factor receptor (EGFR) are made as membrane anchored precursors that can be proteolytically processed and released from the plasma membrane. This process, which is referred to as protein ectodomain shedding, is emerging as a key regulator of the function of EGFR ligands. In light of the important roles of EGFR signaling in development and disease, it will be important to understand more about the regulation of proteolytic processing of EGFR ligands. This chapter describes a sensitive and semiquantitative method to measure ectodomain shedding of EGFR ligands that was designed to facilitate studies of this process in cells.

Original languageEnglish
Pages (from-to)99-113
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume327
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Sahin, U., Weskamp, G., Zheng, Y., Chesneau, V., Horiuchi, K., & Blobel, C. P. (2006). A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor. Methods in molecular biology (Clifton, N.J.), 327, 99-113.