A significant role of stromal fibroblasts in rapidly progressive dry eye in patients with chronic GVHD

Y. Ogawa, K. Yamazaki, M. Kuwana, Y. Mashima, Y. Nakamura, S. Ishida, I. Toda, Y. Oguchi, Kazuo Tsubota, Shinichiro Okamoto, Yutaka Kawakami

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Abstract

PURPOSE. To elucidate histopathologic features of the lacrimal gland in chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. METHODS. Lacrimal gland specimens from five patients who had dry eye as part of the symptoms of chronic GVHD were examined by immunohistochemistry and transmission electron microscopy. Lacrimal gland specimens from five patients with Sjögren's syndrome (SS) were used as control samples. RESULTS. Lymphocytes, predominantly T cells, were found primarily in the periductal areas of the lacrimal gland from patients with chronic GVHD, whereas B cells were the dominant infiltrating cells in the acinar areas of the lacrimal gland from patients with SS. Notable findings in the lacrimal gland from patients with chronic GVHD were marked fibrosis of the glandular interstitium and an increase in the number of CD34+ stromal fibroblasts. These findings were more prominent in patients with severe dry eye than in those with mild dry eye. Electron microscopic observations of the lacrimal gland from patients with chronic GVHD revealed that stromal fibroblasts were attached to various inflammatory cells, especially T cells, through primitive or rudimentary contacts. In addition, the presence of a well-developed rough endoplasmic reticulum in the fibroblasts and newly synthesized collagen fibrils in the extracellular matrix indicated an active production of extracellular matrix components. Electron micrographs revealed multilayered and thickened basal laminae of blood vessels, ducts. and lobules in the lacrimal gland of patients with chronic GVHD; however, these observations were infrequently observed in the lacrimal glands of patients with SS. CONCLUSIONS. The results suggest substantial differences in the lacrimal gland histopathology of patients with chronic GVHD and SS. In addition, it is likely that stromal fibroblasts are actively involved in the pathogenic process of chronic GVHD in the lacrimal gland by producing excessive extracellular matrix components.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume42
Issue number1
Publication statusPublished - 2001

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Lacrimal Apparatus
Graft vs Host Disease
Fibroblasts
Extracellular Matrix
Electrons
T-Lymphocytes
Rough Endoplasmic Reticulum
Acinar Cells
Hematopoietic Stem Cell Transplantation
Transmission Electron Microscopy
Basement Membrane
Blood Vessels
Fibrosis
B-Lymphocytes
Collagen
Immunohistochemistry
Lymphocytes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ogawa, Y., Yamazaki, K., Kuwana, M., Mashima, Y., Nakamura, Y., Ishida, S., ... Kawakami, Y. (2001). A significant role of stromal fibroblasts in rapidly progressive dry eye in patients with chronic GVHD. Investigative Ophthalmology and Visual Science, 42(1), 111-119.

A significant role of stromal fibroblasts in rapidly progressive dry eye in patients with chronic GVHD. / Ogawa, Y.; Yamazaki, K.; Kuwana, M.; Mashima, Y.; Nakamura, Y.; Ishida, S.; Toda, I.; Oguchi, Y.; Tsubota, Kazuo; Okamoto, Shinichiro; Kawakami, Yutaka.

In: Investigative Ophthalmology and Visual Science, Vol. 42, No. 1, 2001, p. 111-119.

Research output: Contribution to journalArticle

Ogawa, Y, Yamazaki, K, Kuwana, M, Mashima, Y, Nakamura, Y, Ishida, S, Toda, I, Oguchi, Y, Tsubota, K, Okamoto, S & Kawakami, Y 2001, 'A significant role of stromal fibroblasts in rapidly progressive dry eye in patients with chronic GVHD', Investigative Ophthalmology and Visual Science, vol. 42, no. 1, pp. 111-119.
Ogawa, Y. ; Yamazaki, K. ; Kuwana, M. ; Mashima, Y. ; Nakamura, Y. ; Ishida, S. ; Toda, I. ; Oguchi, Y. ; Tsubota, Kazuo ; Okamoto, Shinichiro ; Kawakami, Yutaka. / A significant role of stromal fibroblasts in rapidly progressive dry eye in patients with chronic GVHD. In: Investigative Ophthalmology and Visual Science. 2001 ; Vol. 42, No. 1. pp. 111-119.
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T1 - A significant role of stromal fibroblasts in rapidly progressive dry eye in patients with chronic GVHD

AU - Ogawa, Y.

AU - Yamazaki, K.

AU - Kuwana, M.

AU - Mashima, Y.

AU - Nakamura, Y.

AU - Ishida, S.

AU - Toda, I.

AU - Oguchi, Y.

AU - Tsubota, Kazuo

AU - Okamoto, Shinichiro

AU - Kawakami, Yutaka

PY - 2001

Y1 - 2001

N2 - PURPOSE. To elucidate histopathologic features of the lacrimal gland in chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. METHODS. Lacrimal gland specimens from five patients who had dry eye as part of the symptoms of chronic GVHD were examined by immunohistochemistry and transmission electron microscopy. Lacrimal gland specimens from five patients with Sjögren's syndrome (SS) were used as control samples. RESULTS. Lymphocytes, predominantly T cells, were found primarily in the periductal areas of the lacrimal gland from patients with chronic GVHD, whereas B cells were the dominant infiltrating cells in the acinar areas of the lacrimal gland from patients with SS. Notable findings in the lacrimal gland from patients with chronic GVHD were marked fibrosis of the glandular interstitium and an increase in the number of CD34+ stromal fibroblasts. These findings were more prominent in patients with severe dry eye than in those with mild dry eye. Electron microscopic observations of the lacrimal gland from patients with chronic GVHD revealed that stromal fibroblasts were attached to various inflammatory cells, especially T cells, through primitive or rudimentary contacts. In addition, the presence of a well-developed rough endoplasmic reticulum in the fibroblasts and newly synthesized collagen fibrils in the extracellular matrix indicated an active production of extracellular matrix components. Electron micrographs revealed multilayered and thickened basal laminae of blood vessels, ducts. and lobules in the lacrimal gland of patients with chronic GVHD; however, these observations were infrequently observed in the lacrimal glands of patients with SS. CONCLUSIONS. The results suggest substantial differences in the lacrimal gland histopathology of patients with chronic GVHD and SS. In addition, it is likely that stromal fibroblasts are actively involved in the pathogenic process of chronic GVHD in the lacrimal gland by producing excessive extracellular matrix components.

AB - PURPOSE. To elucidate histopathologic features of the lacrimal gland in chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. METHODS. Lacrimal gland specimens from five patients who had dry eye as part of the symptoms of chronic GVHD were examined by immunohistochemistry and transmission electron microscopy. Lacrimal gland specimens from five patients with Sjögren's syndrome (SS) were used as control samples. RESULTS. Lymphocytes, predominantly T cells, were found primarily in the periductal areas of the lacrimal gland from patients with chronic GVHD, whereas B cells were the dominant infiltrating cells in the acinar areas of the lacrimal gland from patients with SS. Notable findings in the lacrimal gland from patients with chronic GVHD were marked fibrosis of the glandular interstitium and an increase in the number of CD34+ stromal fibroblasts. These findings were more prominent in patients with severe dry eye than in those with mild dry eye. Electron microscopic observations of the lacrimal gland from patients with chronic GVHD revealed that stromal fibroblasts were attached to various inflammatory cells, especially T cells, through primitive or rudimentary contacts. In addition, the presence of a well-developed rough endoplasmic reticulum in the fibroblasts and newly synthesized collagen fibrils in the extracellular matrix indicated an active production of extracellular matrix components. Electron micrographs revealed multilayered and thickened basal laminae of blood vessels, ducts. and lobules in the lacrimal gland of patients with chronic GVHD; however, these observations were infrequently observed in the lacrimal glands of patients with SS. CONCLUSIONS. The results suggest substantial differences in the lacrimal gland histopathology of patients with chronic GVHD and SS. In addition, it is likely that stromal fibroblasts are actively involved in the pathogenic process of chronic GVHD in the lacrimal gland by producing excessive extracellular matrix components.

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