A single strain of Clostridium butyricum induces intestinal IL-10-producing macrophages to suppress acute experimental colitis in mice

Atsushi Hayashi, Toshiro Sato, Nobuhiko Kamada, Yohei Mikami, Katsuyoshi Matsuoka, Tadakazu Hisamatsu, Toshifumi Hibi, Axel Roers, Hideo Yagita, Toshiaki Ohteki, Akihiko Yoshimura, Takanori Kanai

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Abstract

Imbalance in gut bacterial composition provokes host proinflammatory responses causing diseases such as colitis. Colonization with a mixture of Clostridium species from clusters IV and XIVa was shown to suppress colitis through the induction of IL-10-producing regulatory T (Treg) cells. We demonstrate that a distinct Clostridium strain from cluster I, Clostridium butyricum (CB), prevents acute experimental colitis in mice through induction of IL-10, an anti-inflammatory cytokine. However, while CB treatment had no effect on IL-10 production by T cells, IL-10-producing F4/80+CD11b +CD11cint macrophages accumulated in the inflamed mucosa after CB treatment. CB directly triggered IL-10 production by intestinal macrophages in inflamed mucosa via the TLR2/MyD88 pathway. The colitis-preventing effect of CB was negated in macrophage-specific IL-10-deficient mice, suggesting that induction of IL-10 by intestinal macrophages is crucial for the probiotic action of CB. Collectively, CB promotes IL-10 production by intestinal macrophages in inflamed mucosa, thereby preventing experimental colitis in mice.

Original languageEnglish
Pages (from-to)711-722
Number of pages12
JournalCell Host and Microbe
Volume13
Issue number6
DOIs
Publication statusPublished - 2013 Jun 12

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Clostridium butyricum
Colitis
Interleukin-10
Macrophages
Mucous Membrane
Clostridium
Regulatory T-Lymphocytes
Probiotics
Anti-Inflammatory Agents
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

A single strain of Clostridium butyricum induces intestinal IL-10-producing macrophages to suppress acute experimental colitis in mice. / Hayashi, Atsushi; Sato, Toshiro; Kamada, Nobuhiko; Mikami, Yohei; Matsuoka, Katsuyoshi; Hisamatsu, Tadakazu; Hibi, Toshifumi; Roers, Axel; Yagita, Hideo; Ohteki, Toshiaki; Yoshimura, Akihiko; Kanai, Takanori.

In: Cell Host and Microbe, Vol. 13, No. 6, 12.06.2013, p. 711-722.

Research output: Contribution to journalArticle

Hayashi, Atsushi ; Sato, Toshiro ; Kamada, Nobuhiko ; Mikami, Yohei ; Matsuoka, Katsuyoshi ; Hisamatsu, Tadakazu ; Hibi, Toshifumi ; Roers, Axel ; Yagita, Hideo ; Ohteki, Toshiaki ; Yoshimura, Akihiko ; Kanai, Takanori. / A single strain of Clostridium butyricum induces intestinal IL-10-producing macrophages to suppress acute experimental colitis in mice. In: Cell Host and Microbe. 2013 ; Vol. 13, No. 6. pp. 711-722.
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