A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila

Lalith S. Gunawardane, Kuniaki Saito, Kazumichi M. Nishida, Keita Miyoshi, Yoshinori Kawamura, Tomoko Nagami, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

726 Citations (Scopus)

Abstract

In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.

Original languageEnglish
Pages (from-to)1587-1590
Number of pages4
JournalScience
Volume315
Issue number5818
DOIs
Publication statusPublished - 2007 Mar 16
Externally publishedYes

Fingerprint

Small Interfering RNA
Drosophila
Solanum melongena
Retroelements
Uracil
Adenine Nucleotides
Germ Cells
Nucleotides

ASJC Scopus subject areas

  • General

Cite this

Gunawardane, L. S., Saito, K., Nishida, K. M., Miyoshi, K., Kawamura, Y., Nagami, T., ... Siomi, M. C. (2007). A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila. Science, 315(5818), 1587-1590. https://doi.org/10.1126/science.1140494

A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila. / Gunawardane, Lalith S.; Saito, Kuniaki; Nishida, Kazumichi M.; Miyoshi, Keita; Kawamura, Yoshinori; Nagami, Tomoko; Siomi, Haruhiko; Siomi, Mikiko C.

In: Science, Vol. 315, No. 5818, 16.03.2007, p. 1587-1590.

Research output: Contribution to journalArticle

Gunawardane, LS, Saito, K, Nishida, KM, Miyoshi, K, Kawamura, Y, Nagami, T, Siomi, H & Siomi, MC 2007, 'A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila', Science, vol. 315, no. 5818, pp. 1587-1590. https://doi.org/10.1126/science.1140494
Gunawardane LS, Saito K, Nishida KM, Miyoshi K, Kawamura Y, Nagami T et al. A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila. Science. 2007 Mar 16;315(5818):1587-1590. https://doi.org/10.1126/science.1140494
Gunawardane, Lalith S. ; Saito, Kuniaki ; Nishida, Kazumichi M. ; Miyoshi, Keita ; Kawamura, Yoshinori ; Nagami, Tomoko ; Siomi, Haruhiko ; Siomi, Mikiko C. / A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila. In: Science. 2007 ; Vol. 315, No. 5818. pp. 1587-1590.
@article{84b88e1578f64a0c9a9f2403cc65c298,
title = "A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila",
abstract = "In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.",
author = "Gunawardane, {Lalith S.} and Kuniaki Saito and Nishida, {Kazumichi M.} and Keita Miyoshi and Yoshinori Kawamura and Tomoko Nagami and Haruhiko Siomi and Siomi, {Mikiko C.}",
year = "2007",
month = "3",
day = "16",
doi = "10.1126/science.1140494",
language = "English",
volume = "315",
pages = "1587--1590",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5818",

}

TY - JOUR

T1 - A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila

AU - Gunawardane, Lalith S.

AU - Saito, Kuniaki

AU - Nishida, Kazumichi M.

AU - Miyoshi, Keita

AU - Kawamura, Yoshinori

AU - Nagami, Tomoko

AU - Siomi, Haruhiko

AU - Siomi, Mikiko C.

PY - 2007/3/16

Y1 - 2007/3/16

N2 - In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.

AB - In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.

UR - http://www.scopus.com/inward/record.url?scp=33947390781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947390781&partnerID=8YFLogxK

U2 - 10.1126/science.1140494

DO - 10.1126/science.1140494

M3 - Article

VL - 315

SP - 1587

EP - 1590

JO - Science

JF - Science

SN - 0036-8075

IS - 5818

ER -