Abstract
In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.
| Original language | English |
|---|---|
| Pages (from-to) | 1587-1590 |
| Number of pages | 4 |
| Journal | Science |
| Volume | 315 |
| Issue number | 5818 |
| DOIs | |
| Publication status | Published - 2007 Mar 16 |
| Externally published | Yes |
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Cite this
A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila. / Gunawardane, Lalith S.; Saito, Kuniaki; Nishida, Kazumichi M.; Miyoshi, Keita; Kawamura, Yoshinori; Nagami, Tomoko; Siomi, Haruhiko; Siomi, Mikiko C.
In: Science, Vol. 315, No. 5818, 16.03.2007, p. 1587-1590.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila
AU - Gunawardane, Lalith S.
AU - Saito, Kuniaki
AU - Nishida, Kazumichi M.
AU - Miyoshi, Keita
AU - Kawamura, Yoshinori
AU - Nagami, Tomoko
AU - Siomi, Haruhiko
AU - Siomi, Mikiko C.
PY - 2007/3/16
Y1 - 2007/3/16
N2 - In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.
AB - In Drosophila, repeat-associated small interfering RNAs (rasiRNAs) are produced in the germ line by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. We sequenced small RNAs associated with the PIWI subfamily member AG03. Although other members of PIWI, Aubergine (Aub) and Piwi, associated with rasiRNAs derived mainly from the antisense strand of retrotransposons, AGO3-associated rasiRNAs arose mainly from the sense strand. Aub- and Piwi-associated rasiRNAs showed a strong preference for uracil at their 5′ ends, and AGO3-associated rasiRNAs showed a strong preference for adenine at nucleotide 10. Comparisons between AGO3- and Aub-associated rasiRNAs revealed pairs of rasiRNAs showing complementarities in their first 10 nucleotides. Aub and AGO3 exhibited Slicer activity in vitro. These data support a model in which formation of a 5′ terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with the Slicer activity of PIWI.
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U2 - 10.1126/science.1140494
DO - 10.1126/science.1140494
M3 - Article
VL - 315
SP - 1587
EP - 1590
JO - Science
JF - Science
SN - 0036-8075
IS - 5818
ER -